| 唐茵茹,姜华,王雪,汪祺,文海若.流式细胞术在遗传毒性评价中的应用[J].中国药事,2022,36(9):1071-1077 |
| 流式细胞术在遗传毒性评价中的应用 |
| Application of Flow Cytometry in Genotoxicity Evaluation |
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| DOI：10.16153/j.1002-7777.2022.09.013 |
| 中文关键词: 流式细胞术 遗传毒性 高通量筛选 微核试验 Pig-a基因突变试验 γH2AX试验 |
| 英文关键词: flow cytometry genotoxicity high-throughput screening micronucleus assay Pig-a gene mutation assay γH2AX assay |
| 基金项目:国家“重大新药创制”科技重大专项(编号 2018ZX09201017)；国家自然科学基金(编号 81503347) |
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| 中文摘要: |
| 遗传毒性是药物临床前安全性评价的重要组成部分，随着新型化合物数量的不断增加，建立高通量筛选方法以评价遗传毒性风险是当前药物研发人员的迫切需求。流式细胞术是一种能在短时间内对细胞进行多参数检测和分选的技术，具有自动化、高通量、特异性强等特点，目前已被应用于多种遗传毒性评价方法中。本文综述了流式细胞术在遗传毒性研究中的应用，并对其在遗传毒性评价上的应用前景进行探讨。 |
| 英文摘要: |
| Genotoxicity is an important part of the preclinical safety evaluation of drugs. With the growing numbers of new compounds, the establishment of high-throughput screening methods to evaluate genotoxicity is an urgent need for drug developers. Flow cytometry is a technology that can perform multi-parameter detection and cell sorting in a short period of time. It has the advantages of automation, high throughput and strong specificity. It has been used in a variety of genotoxicity evaluation. In this paper, the application of flow cytometry in the study of genotoxicity is reviewed, and its application prospect in the evaluation of genotoxicity is discussed. |
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