| 邬伟魁,严倩茹,宋伟.液相色谱检测器联用技术在中药分析中的应用进展[J].中国药事,2022,36(5):569-577 |
| 液相色谱检测器联用技术在中药分析中的应用进展 |
| Progress in the Application of Liquid Chromatography Detector Combination Technology in the Analysis of Chinese Medicine |
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| DOI：10.16153/j.1002-7777.2022.05.011 |
| 中文关键词: 液相色谱 检测器联用 二极管阵列检测器 紫外-可见分光检测器 荧光检测器 蒸发光散射检测器 电喷雾检测器 示差折光检测器 质谱检测器 |
| 英文关键词: liquid chromatography detector combination diode array detector ultraviolet-visible detector fluorescence detector evaporative light scattering detector charged aerosol detector refractive index detector mass spectrometric detector |
| 基金项目:广东省药品监督管理局科技创新项目(编号 2021TDB09) |
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| 中文摘要: |
| 目的：了解液相色谱检测器联用技术在中药分析中的现状，为其进一步应用提供参考。方法： 在中国知网、万方、维普、PubMed等数据库中查找相关文献，梳理中药分析常用检测器联用现状及存在问题。结果与结论：液相系统适配多种检测器，包括紫外-可见分光检测器(UVD)、荧光检测器 (FLD)、蒸发光散射检测器(ELSD)、电喷雾检测器(CAD)、示差折光检测器(RID)、质谱检测器(MSD)等，适用于大部分化合物分析。但是，单一检测器色谱峰容量有限，无法满足中药多组分同时准确测定需求。UVD与ELSD、FLD、CAD、MSD等联用，可同时分析多种不同特性的化合物，提高检测准确度。检测器联用技术已应用于中药含量测定、指纹图谱建立、炮制工艺及血清药物化学研究等方面，为中药分析提供强有力的手段。 |
| 英文摘要: |
| Objective: To understand the current situation of the application of liquid chromatography(LC) detector combination technology in the analysis of Chinese medicine(CM), and provide references for its further application. Methods: Relevant literature was found in databases such as CNKI, Wanfang, VIP and PubMed. The current situation and existing problems of detector combination technology in the analysis of CM were sorted out. Results and Conclusion: LC system is equipped with various detectors, including ultraviolet-visible detector(UVD), fluorescence detector(FLD), evaporative light-scattering detector(ELSD), charged aerosol detector(CAD), refractive index detector(RID) and mass spectrometric detector(MSD), which is suitable for the analysis of most compounds. However, the peak capacity of a single detector is limited, which can not meet the needs of simultaneous and accurate determination of multiple components of CM. UVD combined with ELSD, FLD, CAD and MSD is suitable for the simultaneous determination of a variety of compounds with different characteristics, and can improve the detection accuracy. The detector combination technology has been applied to the content determination of CM, CM fingerprint, CM process of preparing technology and serum pharmacochemistry, which provides a powerful means for the analysis of CM. |
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