| 王福成,李冬华,冯祥瑞,马潇,杨平荣.HPLC-DAD法同时测定七珍汤散（努布炖屯汤）中4个成分[J].中国药事,2020,34(2):222-228 |
| HPLC-DAD法同时测定七珍汤散（努布炖屯汤）中4个成分 |
| Simultaneous Determination of 4 Constituents in Qizhentang San by HPLCDAD |
| 投稿时间：2019-07-30 |
| DOI：10.16153/j.1002-7777.2020.02.011 |
| 中文关键词: 七珍汤散 没食子酸 6-姜辣素 异土木香内酯 土木香内酯 |
| 英文关键词: Qizhentang San Gallic acid 6-Gingerol Isoalantolactone Alantolactone |
| 基金项目:甘肃省科技重大专项（编号17ZD2FA009） |
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| 中文摘要: |
| 目的： 建立HPLC-DAD法同时测定七珍汤散中没食子酸、6-姜辣素、异土木香内酯、土木香内酯的含量，为七珍汤散的质量控制研究、考察其处方各味藏药材的成分和质量标准的提高提供参考。方法： 采用Luna C18色谱柱（4.6 mm×250 mm，5μm），流动相乙腈-0.2%醋酸溶液，梯度洗脱（0~10min，2%→12% A；10~20 min，12%→50% A；20~30 min，50%→85% A；30~50 min，85%→2% A），流速1.0 mL·min-1，柱温35℃，检测波长230、280 nm。结果： 4种成分实现完全分离；没食子酸、6-姜辣素、异土木香内酯、土木香内酯的线性范围依次分别在7.42~148.4、0.92~18.4、1.83~91.25、1.71~85.30 μg（r ≥ 0.9990）；平均加样回收率依次分别为99.06%、99.27%、99.86%、99.36%，RSD依次分别为1.33%、0.67%、0.35%、0.18%。结论： 该方法对4个成分同时测定，简便准确，重复性好，灵敏度高，阴性试验无干扰，可用于七珍汤散的质量控制。 |
| 英文摘要: |
| Objective: To establish an HPLC-DAD method for the simultaneous determination of gallic acid, 6-gingerol, isoalantolactone, alantolactone in Qizhentang San in order to provide references for its quality control. Methods: HPLC separation was performed on a Luna C18 column (4.6 mm×250 mm, 5 μm) with a gradient mobile phase of acetonitrile-0.2% acetic acid delivered at 1.0 mL·min-1. The column temperature was at 35℃ and the detection wavelengths were detected as 230 and 280 nm. Results: Gallic acid, 6-gingerol, isoalantolactone, alantolactone were completely separated from the other constituents. The linear ranges of gallic acid, 6-gingerol, isoalantolactone, lantolactone were 7.42-148.4 μg, 0.92-18.4 μg, 1.83-91.25 μg, and 1.71-85.30 μg (r ≥ 0.9990), respectively. The average recoveries were 99.06%, 99.27%, 99.86% and 99.36% with RSD values at 1.33%, 0.67%, 0.35% and 0.18%, respectively. Conclusion: This method could be used for the simultaneous determination of multi-ingredients in Qizhentang San and the established method was simple and accurate, with good reproducibility and high sensitivity. It could be used for the quality control of Qizhentang San. |
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