| 马玉玲,白瑞斌,郑晓萍,胡林海,孙伯禄,胡芳弟.复方党参口服液的免疫增强功能研究[J].中国药事,2017,(12):1488-1493 |
| 复方党参口服液的免疫增强功能研究 |
| Effect of Complex Dangshen Oral Solution on the Immunological Function of Hypoimmune Mice |
| 投稿时间：2017-01-21 |
| DOI：10.16153/j.1002-7777.2017.12.020 |
| 中文关键词: 复方党参口服液 碳粒廓清试验 迟发型变态反应 脾淋巴细胞增殖转化 |
| 英文关键词: CDOS carbon clearance test delayed type hypersensitivity lymphocyte proliferation |
| 基金项目:甘肃省中医药管理局科研课题（编号GZK-2013-13）；兰州市科技计划项目（编号2013-4-75，214-2-30） |
| 作者 | 单位 | E-mail | | 马玉玲 | 兰州大学 药学院, 兰州 730000, | | | 白瑞斌 | 兰州大学 药学院, 兰州 730000, | | | 郑晓萍 | 兰州大学 药学院, 兰州 730000, | | | 胡林海 | 甘肃省嘉峪关市第一人民医院, 脊柱外科, 嘉峪关 735100 | | | 孙伯禄 | 兰州大学 药学院, 兰州 730000, | | | 胡芳弟 | 兰州大学 药学院, 兰州 730000, | hufd@lzu.edu.cn |
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| 中文摘要: |
| 目的：研究复方党参口服液对环磷酰胺所致免疫低下小鼠免疫功能的影响。方法：昆明种小鼠240只，分为4批，每批60只。每批小鼠随机分为空白组、模型组、阳性药物组、复方党参口服液高、中、低剂量组，每组10只，各组均连续灌胃4周，小鼠于给药3周后开始腹腔注射环磷酰胺40 mg·kg-1，每天1次，连续2 d，建立小鼠免疫力低下模型；通过碳粒廓清试验、迟发型变态反应试验、脾淋巴细胞增殖转化试验、外周血白细胞计数、免疫器官指数五项相关免疫学指标，综合评价复方党参口服液对免疫低下小鼠免疫功能的调节作用。结果：与模型组比较，复方党参口服液可显著提高免疫功能低下小鼠的碳廓清吞噬指数、增强小鼠的迟发型变态反应的DTH程度；对抗环磷酰胺所致的小鼠外周血白细胞数、胸腺指数、脾脏指数以及脾淋巴细胞增殖转化能力的降低，显示出该制剂对免疫低下小鼠单核-巨噬细胞功能、细胞免疫功能的增强作用。结论：复方党参口服液可显著增强免疫低下小鼠的免疫功能，对免疫失衡机体具有一定的调节作用。 |
| 英文摘要: |
| Objective: This paper were designed to explore the effect of Complex Dangshen Oral Solution (CDOS) on the immunological function of immune-suppressed mice induced by cyclophosphamide (CY). Method: 240 Kunming mice were divided into 4 batches, with 60 in each batch. Each batch of mice were randomly divided into six groups as follows:a blank group, a negative control group, a positive control group and CDOS groups with high, middle and low dosages respectively. All the groups received intragastric administration once a day for four weeks. At the beginning of the fourth week, except the blank groups, all the other groups received a daily intraperitoneal injection of CY (40 mg·kg-1) for two days. By the determination of fve immune indexes which were related with carbon clearance test, delayed type hypersensitivity, lymphocyte proliferation test, white blood cells(WBC) and immune organ indexes respectively, a comprehensive evaluation was made on the immunoenhancement effects of CDOS on Hypoimmune Mice. Resuults: Compared with the negative control group, CDOS treatment could obviously improve the phagocytic indexes of mononuclear macrophage in carbon clearance test and the DTH degree in delayed type hypersensitivity. Further more, CDOS had a confrontation effect on the decrease of WBC, thymus index, spleen index and the lymphocyte proliferation ability induced by CY. CDOS presented a significant promotion of mononuclear macrophage and cellular immune function. Conclusion: These results demonstrated that CDOS could enhance the immune function of hypoimmune mice and bring some regulations to the immune imbalance body. |
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