| 王爱娜,冯贞.同时测定小儿咳喘灵颗粒中4种成分的方法研究[J].中国药事,2017,(8):933-939 |
| 同时测定小儿咳喘灵颗粒中4种成分的方法研究 |
| Study on the Method for Simultaneous Determination of Four Components in Xiaoer Kechuanling Granules |
| 投稿时间：2016-11-19 |
| DOI：10.16153/j.1002-7777.2017.08.017 |
| 中文关键词: 中成药 告依春 绿原酸 甘草苷 木犀草苷 高效液相色谱法 |
| 英文关键词: proprietary chinese medicine goitrin chlorogenic acid liquiritin luteoloside HPLC |
| 基金项目: |
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| 摘要点击次数: 1101 |
| 全文下载次数: 486 |
| 中文摘要: |
| 目的：建立同时测定小儿咳喘灵颗粒中（R，S）-告依春、绿原酸、甘草苷和木犀草苷含量的方法。方法：采用高效液相色谱法，Inertsil C8-3色谱柱（4.6 mm×250 mm，5 μm）；流动相：乙腈-0.5%乙酸溶液，梯度洗脱；流速：0.6 mL·min-1；DAD检测器，检测波长：木犀草苷327 nm、绿原酸327nm、甘草苷276 nm、（R，S）-告依春245 nm。结果：金银花中木犀草苷和绿原酸、甘草中甘草苷及板蓝根中（R，S）-告依春在各自的线性范围内与其峰面积呈良好的线性关系；平均加样回收率在99.4%~103.4%范围内，RSD均符合规定。结论：该方法准确、简便、快速，可用于小儿咳喘灵颗粒中多味药材的多个活性成分的含量测定。 |
| 英文摘要: |
| Objective: To establish a method for the simultaneous determination of (R,S)-goitrin, chlorogenic acid, liquiritin and luteoloside in Xiaoer Kechuanling Granules. Methods: HPLC was conducted on a Dikma Inertsil C8-3 column (4.6 mm×250 mm, 5 μm) with the mobile phase of Acetonitrile and 0.5% acetic acid at a flow rate of 0.6 mL·min-1 under gradient elution. Detection wavelengths of DAD detector were as follows:luteoloside (327 nm), chlorogenic acid (327 nm), liquiritin (276 nm), as well as (R, S)-goitrin (245 nm). Results: Luteoloside and chlorogenic acid in honeysuckle, liquiritin in liquorice, and (R, S)-goitrin in radix isatidis all had a good linear relationship with their peak area. The average recoveries were in the range of 99.4%~103.4%. RSD was in accordance with the regulations. Conclusion: The method is accurate, simple, and rapid, and it can be used in the determination of multiple medicinal materials and active components in Xiaoer Kechuanling Granules. |
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