| 张荣林,林雀跃.HPLC法同时测定宫颈炎康栓中苦参碱和槐定碱的含量[J].中国药事,2017,(6):626-631 |
| HPLC法同时测定宫颈炎康栓中苦参碱和槐定碱的含量 |
| Determination of the Content of Matrine and Sophoridine in Gongjing Yankang Suppository Using HPLC Method |
| 投稿时间：2016-12-20 |
| DOI：10.16153/j.1002-7777.2017.06.009 |
| 中文关键词: 高效液相色谱法 宫颈炎康栓 苦参碱 槐定碱 中成药质量控制 |
| 英文关键词: HPLC Gongjing Yankang Suppository matrine sophoridine quality control of Chinese patent medicine |
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| 中文摘要: |
| 目的：建立宫颈炎康栓中苦参碱和槐定碱的HPLC测定方法。方法：采用Ultimate XB-NH2色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-3%磷酸溶液（97︰3），流速为1.0 mL·min-1，检测波长206 nm，柱温35℃。结果：苦参碱进样量在0.05095~2.5475 μg范围内线性关系良好（r=1.0000），平均回收率为98.0%；槐定碱进样量在0.05144~2.5720 μg范围内线性关系良好（r=1.0000），平均回收率为99.9%。结论：本法经方法学验证，可用于中成药宫颈炎康栓的质量控制。 |
| 英文摘要: |
| Objective: To develop HPLC method to determine matrine and sophoridine in Gongjing Yankang Suppository. Methods: The separation was performed on an Ultimate XB-NH2 column (4.6 mm×250 mm, 5 μm) with acetonitrile-3% phosphoric acid (97:3) as mobile phase. The flow rate was 1.0 mL·min-1. The detection UV wavelength was 206 nm. The column temperature was 35℃. Results: The calibration curve of matrine had a good linear relationship in the mass range of 0.05095-2.5475 μg (r=1.0000) and the average recovery rate was 98.0%. The calibration curve of sophoridine also had a good linear relationship in the mass range of 0.05144-2.5720 μg (r=1.0000) and the average recovery rate was 99.9%. Conclusion: The method was validated and could be used for the quality control of Gongjing Yankang Suppository. |
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