| 沙拉麦提·艾力,王亚丽.菟丝草质量标准研究[J].中国药事,2017,(5):501-505 |
| 菟丝草质量标准研究 |
| On Quality Standard of Cuscuta Chinensis |
| 投稿时间：2016-11-22 |
| DOI：10.16153/j.1002-7777.2017.05.007 |
| 中文关键词: 菟丝草 质量标准 薄层色谱法 高效液相色谱法 |
| 英文关键词: cuscuta chinensis quality standard TLC HPLC |
| 基金项目:国家药典委员会国家药品标准提高行动计划项目（编号521） |
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| 中文摘要: |
| 目的：建立菟丝草质量标准。方法：采用TLC法鉴别；采用HPLC法测定菟丝草中金丝桃苷的含量，色谱柱为Agilent Eclipse TC-C18 （250 mm×4.6 mm，5 μm），流动相为乙腈-0.1%磷酸溶液（17∶83），流速为1.0 mL·min-1，柱温为30 ℃，检测波长360 nm。 结果：TLC色谱斑点清晰，金丝桃苷在2.568~206.4 μg·mL-1范围内线性关系良好（r=0.9999），平均回收率为100.18%，RSD为2.09% （n=9） 。结论：本方法准确、简便、灵敏度高，能有效控制菟丝草药材的质量。 |
| 英文摘要: |
| Objective: To establish the quality standard of Cuscuta chinensis. Methods: The cuscuta chinensis was identified with the method of thin-layer chromatography (TLC), and the HPLC method was used to determine the contents of hyperoside in Cuscuta chinensis with an Agilent Eclipse TC-C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid (17∶83), and the flow rate was 1.0 mL·min-1. The detection wavelength was 360 nm, and the column temperature was 30 ℃. Results: TLC spots were clear and well-separated. The calibration curve of hyperoside showed a good linear in the range of 2.568~206.4 μg· mL-1 (r=0.9999) and the average recovery was 100.18% (RSD=2.09%, n=9). Conclusion: The method was accurate, simple, and sensitive, which could be used for the quality control of the cuscuta chinensis. |
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