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| 银翘解毒颗粒中金银花掺伪检查 |
| Experimental Research on the Detection Method of Adulteration of Lonicera Japonica Flos in Yinqiaojiedu Granules |
| 投稿时间：2021-07-07 修订日期：2021-11-23 |
| DOI： |
| 中文关键词: 银翘解毒颗粒 山银花 灰毡毛忍冬皂苷乙 川续断皂苷乙 高效液相色谱-串联质谱法 |
| 英文关键词: Yinqiaojiedu Granules Lonicera Flos macranthoidinSB dipsacoside B LC-MS |
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| 中文摘要: |
| 目的：建立银翘解毒颗粒中金银花掺伪检查方法。方法 采用LC-MS法,色谱柱为Waters CORTECS(150 mm×4.6 mm,2.7 μm),以乙腈和水为流动相进行梯度洗脱,流速为0.5mL.min-1。结果：灰毡毛忍冬皂苷乙在0.0440～5.504μg/ml范围内呈良好的线性关系,平均回收率为101.49%,RSD＝5.97%(n＝9)；川续断皂苷乙在0.0458～5.720μg/ml范围内呈良好的线性关系,平均回收率为104.71%,RSD＝4.99%(n＝9)。结论 本方法灵敏、准确,可作为银翘解毒颗粒中金银花掺伪检查方法。 |
| 英文摘要: |
| Objective：To establish the identification method of Lonicera japonica in Yinqiaojiedu Granules. Methods： LC-MS method was used with Waters CORTECS(150 mm×4.6 mm,2.7 μm) as analytical column, acetonitrile and water was used as mobile phase for gradient elution, the flow rate was 0.5 mL.min-1. Results：The calibration curve was linear in the range of 0.0440～5.504μg/ml for macranthoidinSB, and the average recovery was 101.49% with RSD＝5.97%(n＝9); the calibration curve was linear in the range of 0.0458～5.720μg/ml for dipsacoside B, and the average recovery was 104.71% with RSD＝4.99%(n＝9). Conclusion：The method is accurate and reliable, which can be applied to quality control of Lonicera japonica in Yinqiaojiedu Granules. |
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