| 娜仁图雅,籍学伟,韩塔娜,高磊,郑健.蒙药材蓝盆花指纹图谱及一测多评含量测定方法研究[J].中国药事,2020,34(2):184-194 |
| 蒙药材蓝盆花指纹图谱及一测多评含量测定方法研究 |
| Combinational Quality Control Method of Mongolian Medicinal Materials Flos Scabiosae Based on Fingerprint and QAMS |
| 投稿时间：2019-07-30 |
| DOI：10.16153/j.1002-7777.2020.02.007 |
| 中文关键词: 蓝盆花 指纹图谱 一测多评法 绿原酸 木犀草苷 异绿原酸A 异绿原酸C 高效液相色谱法 |
| 英文关键词: Flos Scabiosae fingerprint quantitative analysis of multi-components by single marker (QAMS) chlorogenic acid galuteolin Isochlorogenic acid A Isochlorogenic acid C HPLC |
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| 中文摘要: |
| 目的： 建立蒙药材蓝盆花的HPLC指纹图谱及一测多评法同时测定绿原酸、木犀草苷、异绿原酸A及异绿原酸C 4个成分的含量，评价药材质量。方法： 采用Kromasil 100-5-C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.2%磷酸溶液为流动相，梯度洗脱，流速1.0 mL·min-1，检测波长分别为326 nm（含量测定）和350 nm（指纹图谱），柱温30℃。以17批样品建立指纹图谱共有模式。以异绿原酸A为内参物，建立绿原酸、木犀草苷、异绿原酸C与内参物的相对校正因子，计算含量，并与外标法计算结果进行比较。结果： 建立了蓝盆花药材的HPLC指纹图谱，标示了10个共有峰，并指认其中的7个色谱峰，分别为绿原酸、木犀草苷、异绿原酸A、大波斯菊苷、异绿原酸C、木犀草素、芹菜素。相对校正因子重现性良好，以异绿原酸A为内参物采用校正因子计算的含量值与实测值之间无显著差异。结论： 采用指纹图谱与多指标检测模式可为评价蒙药材蓝盆花的质量提供有效方法。 |
| 英文摘要: |
| Objective: To establish a quality control method of Flos Scabiosaeby HPLC fingerprint and simultaneous determination of chlorogenic acid, galuteolin, isochlorogenic acid A and isochlorogenic acid C by the quantitative analysis of multi-components by single marker (QAMS) for evaluation of medicinal materials quality. Methods: Kromasil 100-5-C18 column (250 mm×4.6 mm, 5 μm) was employed with a mobile phase of a cetonitrile-0.2% phosphoric acid solution with gradient elution. Detection wavelength was set at 350 nm (fingerprint) and 326 nm(QAMS), the column temperature was 30℃, the flow rate was 1.0 mL·min-1, and the injection volume was 20 μL. The common mode of fingerprint was established using the determination of seventeen batches of Scabiosa Flos. Using isochlorogenic acid A as an internal reference, the relative correction factors among chlorogenic acid, galuteolin, isochlorogenic acid C and the internal reference were established, and the contents were calculated and compared to those obtained with external standard method. Results: HPLC fingerprint was established for the medicinal materials of Scabiosa Flos, 10 common peaks were labeled, and 7 chromatographic peaks were identified, which were chlorogenic acid, galuteolin, isochlorogenic acid A, apigenin-7-O-glucoside, isochlorogenic acid C, luteolin and apigenin, respectively. The established relative correction factors (RCF) had good reproducibility. No significant difference was found between the quantitative results of external standard method and QAMS. Conclusion: The application of HPLC fingerprint and QAMS method model can provide an effective method to evaluate the quality of Scabiosa Flos. |
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