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| 基于网络药理学和分子对接技术探讨连陈汤治疗痰湿型肥胖的作用机制 |
| To explore the mechanism of Lianchen Decoction in treating phlegm-dampness obesity based on network pharmacology and molecular docking technology |
| 投稿时间：2024-06-20 修订日期：2024-08-21 |
| DOI： |
| 中文关键词: 肥胖 痰湿型 连陈汤 网络药理学 分子对接 |
| 英文关键词: obesity Phlegm-dampness type Lianchen Decoction Network pharmacology Molecular docking |
| 基金项目:黑龙江省自然科学基金项目计划任务书 |
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| 中文摘要: |
| 目的：基于网络药理学和分子对接技术探讨连陈汤治疗痰湿型肥胖的作用机制。方法：运用中药系统药理学数据库与分析平台（TCMSP）获取连陈汤有效活性成分及作用靶点。通过人类基因组数据库（GeneCards）、药物靶标数据库（TTD）和在线人类孟德尔遗传数据库（OMIMO）获取疾病靶点。通过证候本体数据库及多维定量关联计算平台（SoFDA）获取痰湿型相关靶点。通过Cytoscape 3.10.1软件构建网络，拓扑分析筛选关键活性成分、核心靶点。使用微生信平台对潜在作用靶点进行GO功能富集和KEGG信号通路富集分析。利用AutoDockVina软件对成分和靶点进行分子对接。结果：获得连陈汤靶点148个、疾病靶点9958个、痰湿型靶点896个。将靶点取交集，获得潜在作用靶点106个。网络拓扑分析获得甘油、米桔素、穆坪马兜铃酰胺为关键活性成分。PPI网络分析筛选出MC4R、PPARG、FTO、GHRL等活跃的靶点。GO功能富集分析显示，包括生物学过程、细胞组件及分子功能3个部分。EGG通路富集分析显示，靶点主要富集在胰岛素抵抗等相关通路。结论：连陈汤可能通过甘油、米桔素、穆坪马兜铃酰胺等关键活性成分作用于MC4R、PPARG、FTO、GHRL等核心靶点调控胰岛素抵抗通路、胰岛素信号通路等信号通路发挥治疗痰湿型肥胖的作用。 |
| 英文摘要: |
| Objective: To explore the mechanism of Lianchen Decoction in treating phlegm-dampness obesity based on network pharmacology and molecular docking technology. Methods: TCMSP was used to obtain the active ingredients and target of Lianchen Decoction. Disease targets were obtained through GeneCards, TTD, and OMIM. The related targets of phlegm-dampness type were obtained by SoFDA. The network is constructed using Cytoscape3.10.1 software to screen key active ingredients and core targets through topological analysis. GO function enrichment and KEGG signaling pathway enrichment analysis were performed on potential targets using Weisheng platform. AutoDockVina software was used for molecular docking of components and targets. Results: 148 targets, 9958 targets of disease and 896 targets of phlegm-dampness were obtained. The targets were intersected and 106 potential targets were obtained. The network topology analysis showed that glycerol, citrin and mupingaristolamide were the key active components. PPI network analysis screened out MC4R, PPARG, FTO, GHRL and other active targets. GO functional enrichment analysis showed that there were three parts: biological process, cell components and molecular function. KEGG pathway enrichment analysis showed that the target was mainly concentrated in insulin resistance related pathways. Conclusion: Lianchen Decoction may act on MC4R, PPARG, FTO, GHRL and other core targets to regulate insulin resistance pathway and insulin signaling pathway through the action of key active ingredients such as glycerol, oryzolin, Mupingaristolamide, and play a role in the treatment of phlegm and dampness type obesity. |
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