文章摘要
汤俊,陈翔,徐玲,胡敏,肖凌.基于网络药理学对鹿蹄橐吾镇咳活性成分的初步研究[J].中国药事,2024,38(4):423-438
基于网络药理学对鹿蹄橐吾镇咳活性成分的初步研究
Preliminary Study on the Anti-cough Active Components of Ligularia hodgsonii Hook. Based on Network Pharmacology
投稿时间：2023-10-22  
DOI：10.16153/j.1002-7777.2024.04.008
中文关键词: 鹿蹄橐吾  镇咳  网络药理学  活性成分  分子机制
英文关键词: Ligularia hodgsonii Hook.  cough-relieving effect  network pharmacology  active ingredients  molecular mechanism
基金项目:国家自然科学基金项目（编号 31970349）；湖北省重点研发计划项目（编号 2020ACA007-01）
作者单位
汤俊 武汉大学药学院，组合生物合成与新药发现教育部重点实验室，武汉 430071 
陈翔 武汉大学药学院，组合生物合成与新药发现教育部重点实验室，武汉 430071 
徐玲 湖北省药品监督检验研究院，国家药品监督管理局中药质量控制重点实验室，湖北省药品质量检测与控制工程技术研究中心，武汉 430075 
胡敏 湖北省药品监督检验研究院，国家药品监督管理局中药质量控制重点实验室，湖北省药品质量检测与控制工程技术研究中心，武汉 430075 
肖凌 湖北省药品监督检验研究院，国家药品监督管理局中药质量控制重点实验室，湖北省药品质量检测与控制工程技术研究中心，武汉 430075 
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中文摘要:
      目的：采用网络药理学方法获取鹿蹄橐吾治疗咳嗽的潜在活性成分及蛋白靶点，并探讨可能的作用机制。方法：从PubMed等数据平台获得鹿蹄橐吾化学成分并使用SwissADME和SwissTargetprediction 对其进行筛选，获取潜在成分；利用GeneCards获取咳嗽相关靶点；通过STRING和Cytoscape构建和分析PPI网络，获取核心靶点；使用Metascape平台进行GO生物功能过程和KEGG代谢通路富集分析。结果：在多平台分析下得到23个核心靶点，其对应成分中多为艾里莫芬烷型倍半萜，GO分析获取了如蛋白磷酸化正调控、炎症反应调节等生物过程，细胞膜及突触等细胞组成，以及蛋白激酶结合、蛋白质结构域特异性结合等分子功能条目。KEGG富集显示与炎症等相关的信号通路。结论：鹿蹄橐吾存在改善咳嗽病征的有效成分，且以多组分、多靶点、多通路的形式发挥作用，其机制可能涉及炎症和免疫等相关通路。
英文摘要:
      Objective: To obtain the potential active ingredients and protein targets of Ligularia hodgsonii Hook. in the treatment of cough, and to explore the possible mechanism of action by Network Pharmacology. Methods: The chemical constituents of Ligularia hodgsonii Hook. were retrieved from PubMed and other data platforms, then the potential targets screened out through SwissADME and SwissTargetprediction. Cough related targets were obtained by using GeneCards. Key targets were obtained from STRING and Cytoscape, which were used to construct and analyze PPI network. Metascape platform was used for GO biological function process and KEGG metabolic pathway enrichment analysis. Results: Total of 23 key targets was obtained by multi-platform analysis, and most of the corresponding components were eremophilane-type sesquiterpenes. GO analysis obtained biological processes such as positive regulation of protein phosphorylation and regulation of inflammatory response, cellular component such as cell membrane and synapse, molecular function such as protein kinase binding and protein domain specifi c binding. The results of KEGG enrichment showed the signal pathways related to infl ammation.Conclusion: Ligularia hodgsonii Hook. has many active ingredients, which may improve cough symptoms and functions via multi-component multi-target manner and multi-pathway. The underlying mechanism may involve related pathways of infl ammation and immunity.
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