| Objective: Establish a method for detecting red ginseng adulterated with steamed American ginseng in Dahuoluo Pills to provide an important support for supervising its quality. Method: Taking pseudo-ginsenoside F11, the characteristic component of American ginseng, as the adulteration detection index, an UPLC-MSMS analysis was performed on C18 column (100 mm×2.1 mm, 1.8 μm), using acetonitrile and 10 mmol.L-1 ammonium formate solution as mobile phase for gradient elution at a ?ow rate 0.3 mL.min-1, and column temperature was 40 ℃. The analyte was detected by electrospray anion scanning and multiple reaction monitoring mode. Results: The preparation has a non-obvious matrix effect; pseudo-ginsenoside F11 showed a good linearity in the range of 0.0117~1.167 μg.mL-1 (R2＞0.999), and also has a linear relationship with different adulteration proportion. The average recovery was 95.2% with RSD=1.7% (n=6). Among the 98 samples, pseudo-ginsenoside F11 was detected in 37 samples, and 5 samples from an enterprise exceededSthe proposed detection limit, leading to the disqualified rate was 5.1%. Conclusion: Compared to the reported literatures, this study launched the supplementary testing method more comprehensively, and the established method was sensitive and accurate, which can be applied to be a supplementary inspection of Dahuoluo Pills. |