| 迪力努尔·艾尼,阿依提拉·麦麦提,康小龙.刺山柑乙醇提取物乳膏对系统性硬皮病小鼠Notch1、DLL1和ADAM17表达的影响[J].中国药事,2023,(4):420-425 |
| 刺山柑乙醇提取物乳膏对系统性硬皮病小鼠Notch1、DLL1和ADAM17表达的影响 |
| Eff ects of Capparis Spinosa Ethanolic Extract Cream on Expressions of Notch1, DLL1 and ADAM17 in Systemic Sclerosis Mice |
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| DOI：10.16153/j.1002-7777.2023.04.008 |
| 中文关键词: 刺山柑乙醇提取物乳膏 系统性硬皮病 Notch1 DLL1 ADAM17 |
| 英文关键词: capparis spinosa ethanolic extract cream systemic sclerosis Notch1 DLL1 ADAM17 |
| 基金项目:新疆维吾尔自治区自然科学基金(编号 2022D01C557) |
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| 中文摘要: |
| 目的：在系统性硬皮病(SSc)模型小鼠上研究刺山柑乙醇提取物乳膏对Notch通路中重要蛋白 Notch1、DLL1和ADAM17的调控作用。方法：给予刺山柑乙醇提取物乳膏冶疗SSc模型小鼠(模型应用 BALB/c小鼠皮下注射博莱霉素4 w建立)8 w。小鼠皮肤组织中Notch1 mRNA和蛋白的表达分别采用实时荧光逆转录PCR(qPCR)和免疫组化法测定，DLL1和ADAM17含量采用ELISA法测定。结果：过表达的Notch1 mRNA和蛋白可被450、900 mg·kg-1刺山柑乙醇提取物乳膏抑制，DLL1和ADAM17可被900 mg·kg-1刺山柑乙醇提取物乳膏抑制。结论：SSc小鼠受损皮肤Notch通路中过表达的Notch1、DLL1和 ADAM17可被刺山柑乙醇提取物乳膏抑制，提示其对SSc Notch通路的过度激活有一定抑制作用。 |
| 英文摘要: |
| Objective: To explore the regulation eff ects of capparis spinosa ethanolic extract cream on expressions of Notch1, DLL1 and ADAM17 in Notch pathway in Systemic Sclerosis (SSc) mice. Methods: SSc modelmice were treated with capparis spinosa ethanolic extract cream (the model was established by subcutaneous injection of bleomycin for 4 w in BALB/c mice) for 8 w. The mRNA expression of Notch1 and protein in mouse skin were detected by real-time reverse transcriptase PCR (qPCR) and immunohistochemical method, respectively. The contents of DLL1 and ADAM17 were measured by ELISA in skin tissue. Results: The mRNA and protein expression of Notch1 were inhibited by 450 and 900 mg·kg-1 capparis spinosa ethanolic extract cream and DLL1 and ADAM17 were inhibited by 900 mg·kg-1 capparis spinosa ethanolic extract cream. Conclusion: Capparis spinosa ethanolic extract cream could inhibit the Notch pathway in damaged skin of SSc mice by down-regulating the expressions of Notch1, DLL1 and ADAM17, suggesting that the SSc Notch pathway overactivation could be inhibited to some extent. |
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