| 董姣姣,杨江威,赵卓,王金恒.支原体液体培养基质控菌生长分析[J].中国药事,2023,(2):210-214 |
| 支原体液体培养基质控菌生长分析 |
| Growth Analysis of Quality Control Strains in Mycoplasma Liquid Culture Medium |
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| DOI：10.16153/j.1002-7777.2023.02.013 |
| 中文关键词: 质控菌 生长曲线 颜色变化单位 支原体培养基 有效期 |
| 英文关键词: quality control strains growth curve color change unit (CCU) mycoplasma medium validity period |
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| 中文摘要: |
| 目的：摸索肺炎支原体在支原体肉汤培养基和口腔支原体在精氨酸支原体肉汤培养基中的生长规律，比较不同密闭方式对支原体液体培养基灵敏度的影响，同时考察液体培养基基础的有效期。方法： 采用变色单位试验法测试肺炎支原体和口腔支原体在相应液体培养基不同培养时间段的颜色变化单位 (CCU)，并绘制生长曲线。使用3种不同密闭方式的器具制备液体培养基并检测灵敏度。测试液体培养基基础存放0、15、30、60和90天的灵敏度。结果：使用3种不同密闭方式培养，支原体肉汤培养基和精氨酸支原体肉汤培养基灵敏度均达到109 CCU·mL-1。支原体肉汤基础和精氨酸支原体肉汤基础存放于2~8 ℃有效期为90天。结论：本试验为支原体液体培养基灵敏度的检查和研究提供依据。 |
| 英文摘要: |
| Objective: To explore the growth regularity of Mycoplasma pneumoniae in mycoplasma broth medium and Mycoplasma oral in arginine mycoplasma broth medium, compare the eff ects of diff erent airtight ways on the sensitivity of mycoplasma liquid medium, and investigate the storage validity period of prepared liquid basic medium. Methods: The color change unit (CCU) of Mycoplasma pneumoniae and Mycoplasma oral in the diff erent growth stages was measured by the color change unit test, and their growth curve was drawn. The liquid culture medium was prepared by using three different sealed instruments and the sensitivity was tested. The sensitivity of prepared liquid basic medium was tested at 0, 15, 30, 60 and 90 days. Results: The sensitivity of both mycoplasma broth medium and arginine mycoplasma broth medium reached 109 CCU·mL-1 in tightly stoppered containers with three kinds of instruments. The validity period of mycoplasma broth and arginine mycoplasma broth basic medium was 90 days at 2-8 ℃. Conclusion: This experiment provided a reference for the test and study of the sensitivity of mycoplasma liquid medium. |
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