| 苏媛,刘安平,刘学良,李维业,韩天楚,杨增亮,王开祥,刘海青.常用“蒂达”类藏药材四数獐牙菜的成分分析[J].中国药事,2022,36(11):1280-1285 |
| 常用“蒂达”类藏药材四数獐牙菜的成分分析 |
| Component Analysis of Swertiatetraptera |
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| DOI：10.16153/j.1002-7777.2022.11.010 |
| 中文关键词: 四数獐牙菜 高效液相色谱法 獐牙菜苦苷 龙胆苦苷 当药苷 当药醇苷 1-羟基-3 4 5三甲氧基三酮 含量测定 |
| 英文关键词: Swertiatetraptera HPLC Swertiamarin Gentiopicrin Sweroside Dangyao alcohol glycoside 1-hydroxy-3 4 5 trimethoxydione content determination |
| 基金项目:青海省基础研究项目(编号 2020-ZJ747) |
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| 中文摘要: |
| 目的：建立高效液相色谱法同时测定四数獐牙菜中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮含量的方法。方法：采用Waters SunFire C18柱(4.6 mm×250 mm， 5 µm)；以0.05%磷酸溶液-甲醇为流动相进行梯度洗脱；柱温30 ℃，流速1.0 mL·min-1，检测波长254 nm。结果：獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮分别在 0.0320~0.3202μg(r=0.9997)、0.3788~3.7881μg(r=0.9998)、0.0124~0.1237μg(r=0.9995)、 0.0032~0.0321μg(r=0.9998)、0.0031~0.0308μg(r=0.9994)进样质量范围内呈良好的线性关系， 加样回收率分别为98.69%、97.81%、92.21%、93.44%、92.77%，RSD(r=6)分别为0.76%、1.77%、 1.98%、1.59%、1.79%。7批样品中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮的测定结果分别为0.1924%~0.3740%、2.1426%~4.4514%、0.1122%~0.1973%、0.0286%~0.0339%及 0.0043%~0.0938%。结论：该方法简便、高效、灵敏、准确、经济实用，具有良好的重复性和稳定性， 对于四数獐牙菜的质量控制、综合利用提供了有效参考依据。 |
| 英文摘要: |
| Objective: To establish a HPLC method for the simultaneous determination of Swertiamarin, Gentiopicrin, Sweroside, Dangyao alcohol glycoside and 1-hydroxy-3,4,5 trimethoxydione in Swertiatetraptera. Methods: Waters Sunfire C18 column (4.6 mm × 250 mm, 5 µm) was used; gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase; the column temperature was 30 ℃, the flow rate was 1.0 mL·min-1, and the detection wavelength was 254 nm. Results: Five components have shown good linear relationship within the injection quality ranges of 0.0320-0.3202 μg (r=0.9997), 0.3788-3.7881 μg (r=0.9998), 0.0124-0.1237 μg (r=0.9995), 0.0032-0.0321 μg (r=0.9998) and 0.0031-0.0308 μg (r=0.9994) respectively. The recovery rates were 98.69%, 97.81%, 92.21%, 93.44% and 92.77% (n=6), and RSDs (r=6) were 0.76%, 1.77%, 1.98%, 1.59% and 1.79%. The results of the determination of the five components in 7 batches of samples were 0.1924%-0.3740%, 2.1426%-4.4514%, 0.1122%-0.1973%, 0.0286%-0.0339% and 0.0043%-0.0938% respectively. Conclusion: The method is simple, efficient, sensitive, accurate, economical and practical with good repeatability and stability. It provides an effective reference for the quality control and comprehensive utilization of Swertiatetraptera. |
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