| 雒磊,任晓东,马文兵,姚鸿萍.促红细胞生成素对高脂血症大鼠的降脂作用及机制研究[J].中国药事,2022,36(10):1166-1173 |
| 促红细胞生成素对高脂血症大鼠的降脂作用及机制研究 |
| The Lipid-lowering Eff ect and Mechanism of Erythropoietin on Hyperlipidemia Rats |
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| DOI：10.16153/j.1002-7777.2022.10.008 |
| 中文关键词: 促红细胞生成素 高脂血症 血液黏度 氧化损伤 炎症 |
| 英文关键词: erythropoietin hyperlipidemia blood viscosity oxidative damage infl ammatory response |
| 基金项目:陕西省自然科学基金(编号 2015JM8447) |
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| 中文摘要: |
| 目的：探讨促红细胞生成素(EPO)对高脂血症大鼠血脂水平的调节及作用机制。方法：将SD 大鼠随机分为正常对照组、高脂血症模型组、EPO组，每组8只。正常对照组大鼠给予基础饲料，模型组及EPO组大鼠给予高脂饲料，连续喂养8周。8周后EPO组按1000 U·kg-1腹腔注射EPO，每周3次，正常对照组和高脂血症模型组大鼠腹腔注射生理盐水。给药4周后，检测血清总胆固醇(TC)、甘油三酯 (TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平及炎症因子白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)含量，肝脏组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性，血液流变学检测血液黏度相关指标，蛋白免疫印迹(Western Blotting)检测肝脏组织SOD1、IL-1β的蛋白表达。结果：EPO不影响高脂血症大鼠体质量增长，可显著降低高脂血症大鼠TC、TG、LDL-C水平，升高HDL-C水平；EPO可改善高脂血症大鼠血液黏滞性，降低低切变率、 中切变率、高切变率全血黏度和中切变率全血还原黏度、血沉等指标；还可明显降低高脂血症大鼠肝脏组织MDA含量，升高SOD活性，降低血清IL-1β和TNF-α水平，增强肝脏组织SOD1蛋白表达，减弱 IL-1β蛋白表达。结论：EPO可显著改善高脂血症大鼠的血脂水平，其降脂作用机制可能与改善血液黏滞性、减轻肝脏氧化损伤和炎症反应有关。 |
| 英文摘要: |
| Objective: To investigate the regulation and mechanism of erythropoietin (EPO) on blood lipid level in hyperlipidemia rats. Methods: SD rats were randomly divided into control group, hyperlipidemia model group and EPO group, with 8 rats in each group. Basic diet was provided to the rats in control group, and high-fat diet was provided to the rats in model group and EPO group for 8 weeks. After 8 weeks, the rats in EPO group were intraperitoneally injected with 1000 U∙kg-1 EPO three times per week, and the rats in control group and hyperlipidemia model group were intraperitoneally injected with normal saline. Subsequent to 4 Objective: To investigate the regulation and mechanism of erythropoietin (EPO) on blood lipid level in hyperlipidemia rats. Methods: SD rats were randomly divided into control group, hyperlipidemia model group and EPO group, with 8 rats in each group. Basic diet was provided to the rats in control group, and high-fat diet was provided to the rats in model group and EPO group for 8 weeks. After 8 weeks, the rats in EPO group were intraperitoneally injected with 1000 U∙kg-1 EPO three times per week, and the rats in control group and hyperlipidemia model group were intraperitoneally injected with normal saline. Subsequent to 4 |
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