| 柴国林,宋平顺,魏玉萍,郑检,孙科.基于HPLC技术的前胡与硬前胡真伪鉴别和掺假的方法研究[J].中国药事,2022,36(9):1040-1048 |
| 基于HPLC技术的前胡与硬前胡真伪鉴别和掺假的方法研究 |
| Research on Adulteration Methods and Identification of Peucedani Radix and Peucedani Subglabri Radix Based on HPLC Technology |
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| DOI：10.16153/j.1002-7777.2022.09.010 |
| 中文关键词: 前胡 硬前胡 高效液相色谱 峰面积比值法 指纹图谱 鉴别 |
| 英文关键词: Peucedani Radix Peucedani Subglabri Radix HPLC peak area ratio method fingerprint identification |
| 基金项目:中医药现代化研究(编号 2019YFC1711505)；甘肃省重点研发(编号 2021GSMPA-KL01、2021GSMPA-KL02) |
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| 中文摘要: |
| 目的：建立前胡中掺假硬前胡的专属性鉴别方法，为前胡药材的质量监控和进一步的补充检验方法提供科学依据。方法：采用Waters C18色谱柱(250 mm×4.60 mm，5 μm)，以甲醇(A)- 水(B) 为流动相，梯度洗脱，流速 1.0 mL·min-1，检测波长 320 nm，柱温 30 ℃，进样体积 10 µL，建立 HPLC技术的特征峰面积比值法和指纹图谱法，对于前胡与硬前胡两种药材或饮片的真伪性进行鉴别。 结果：指纹图谱相似度分析，前胡相似度为0.981~0.998，硬前胡相似度为0.103~0.169，实现了二者的完全鉴别；通过44批次前胡中掺假硬前胡的样品分析，特征峰比1和峰比2分别实现了95.6%和97.8%的鉴别，特征峰比适用于掺假判断。结论：该方法精密度良好，重复性较好，试验分离度高，为前胡药材质量控制、真伪鉴别及掺假检验提供专属性强的方法，能有效提高前胡相关产品用药安全。 |
| 英文摘要: |
| Objective: To establish a specific identification method of adulterating Peucedani Subglabri Radix in Peucedani Radix and lay a scientific basis for the quality control and further supplementary inspection methods of Peucedani Radix medicinal materials. Methods: Waters C18 column (250 mm× 4.60 mm, 5 μm) was used with methanol (A)-water(B) as mobile phase by gradient elution. The flow rate was 1.0 mL·min-1, the detection wavelength was 320 nm, the column temperature was 30 ℃, and the injection volume was 10 µL. The characteristic peak area ratio method and fingerprint method of HPLC technology were established to identify the authenticity of two kinds of medicinal materials or decoction pieces of Peucedani Radix and Peucedani Subglabri Radix. Results: Fingerprint similarity analysis showed that the similarities of Peucedani Radix and Peucedani Subglabri Radix were 0.981-0.998 and 0.103-0.169, respectively, which realized the identification of the two. Through the analysis of 44 batches of samples adulterated with Peucedani Subglabri Radix, the identification of characteristic peak ratio 1 and peak ratio 2 achieved 95.6% and 97.8%, respectively, and the characteristic peak ratio was suitable for adulteration judgment. Conclusion: This method has good precision, good repeatability and high separation. It provides a specific method for the quality control, authenticity identification and adulteration test of Peucedani Radix medicinal materials and can effectively improve the medication safety of Peucedani Radix related products. |
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