文章摘要
曹阳洋,张亚杰,崔方,李文,王楠,徐巧红,胡芳弟.基于HPLC-DAD指纹图谱和化学计量法分析的红药子抗氧化活性谱效关联研究[J].中国药事,2022,36(8):943-953
基于HPLC-DAD指纹图谱和化学计量法分析的红药子抗氧化活性谱效关联研究
Study on Spectral-Eff ect Antioxidant Activity Profi les of Rodgersia Aesculifolia Batalin Based on HPLC-DAD Fingerprinting and Stoichiometric Analysis
  
DOI：10.16153/j.1002-7777.2022.08.012
中文关键词: 红药子  指纹图谱  抗氧化活性  谱效关系  化学计量法
英文关键词: Rodgersia aesculifolia Batalin  fingerprint spectrums  antioxidant activity  spectrum-effect relationship  Stoichiometry
基金项目:甘肃省重大项目(212D4FA013)；“十三五”国家重点研发计划重点专项(编号 2018YFC1706300、2018YFC17063003)；甘肃省技术创新引导计划-民生专项(编号 20CX4FK014)；兰州市人才创新创业项目(编号2017-RC115、2020-RC-41)
作者单位
曹阳洋 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
张亚杰 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
崔方 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
李文 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
王楠 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
徐巧红 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
胡芳弟 兰州大学药学院,功能有机分子化学国家重点实验室,兰州大学陇药协同创新中心,兰州 730000 
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中文摘要:
      目的：建立红药子药材醇提物的HPLC-DAD指纹图谱，分析指纹图谱与抗氧化作用的关联性， 挖掘红药子抗氧化的物质基础，为控制红药子的质量提供依据。方法：通过HPLC-DAD法建立10批红药子醇提物的指纹图谱，以1,1-二苯基-2-三硝基苯肼自由基(DPPH)清除活性、超氧阴离子自由基清除活性、羟自由基清除活性为指标评价红药子药材的抗氧化活性，并采用偏最小二乘法(PLS)分析 HPLC-DAD指纹图谱与抗氧化活性关系，筛选红药子药材指标性成分。结果：10批红药子的指纹图谱共匹配出21个共有峰，其相似度均大于0.90。通过化学计量学方法分析，综合相关系数和VIP值两个指标， 确定9个共有峰与DPPH自由基清除活性呈正相关且VIP值均大于1，3个共有峰与超氧阴离子清除活性呈正相关且VIP值均大于1，8个共有峰与羟自由基清除活性呈正相关且VIP值均大于1。最后经HPLC-QTOF-MS鉴别了红药子药材指纹图谱中10个峰，共鉴定出7个共有特征峰，3个非共有峰。其中，与抗氧化活性最相关的三个峰10、12、14分别为绿原酸、岩白菜素、隐绿原酸。结论：建立的红药子药材的指纹图谱方法简单可行，通过谱效关系分析筛选出抗氧化活性的重要成分，为明确红药子抗氧化作用提供物质基础，为整体控制红药子质量提供依据。
英文摘要:
      Objective: To establish a HPLC-DAD fi ngerprint spectrum for the alcohol extraction of Rodgersia aesculifolia Batalin, analyze the correlation between the fi ngerprint spectrum and the antioxidant eff ect, explore the antioxidant material basis of Rodgersia aesculifolia Batalin in order to lay a basis for the quality control of Rodgersia aesculifolia Batalin. Methods: Fingerprint spectrums of alcohol extraction from 10 batches of Rodgersia aesculifolia Batalin were established by using the HPLC-DAD method. The antioxidant activity of Rodgersia aesculifolia Batalin were evaluated based on DPPH radical scavenging activity, superoxide anion radical (O2-) scavenging activity, and hydroxyl radical (-OH) scavenging activity. The relationship between HPLC-DAD fi ngerprint spectrums and antioxidant activity was analyzed by PLS, and the index components of Rodgersia aesculifolia Batalin were screened. Results: 21 common peaks were matched from the fi ngerprint spectrums of 10 batches of Rodgersia aesculifolia Batalin and their similarities were all greater than 0.90. Through the chemometric analysis, the comprehensive correlation coeffi cient and VIP value of two indicators, 9 common peaks were determined in positive correlation with DPPH free radical scavenging activity and the VIP values were greater than 1. 3 common peaks were positively correlated with superoxide anion scavenging activity and the VIP values were greater than 1. 8 common peaks were positively correlated with hydroxyl radical scavenging activity and the VIP values were greater than 1. Finally, 10 peaks in the fi ngerprint spectrum of Rodgersia aesculifolia Batalin were identifi ed by HPLC-Q-TOF-MS with 7 peaks of common characteristics and 3 non-common peaks. Among them, the three peaks (10, 12 and 14) most related to the antioxidant activity were chlorogenic acid, bergenin and cryptochlorogenic acid. Conclusion: The established method for the fi ngerprint spectrum of Rodgersia aesculifolia Batalin is simple and feasible, and the important components of antioxidant activity were screened out by the analysis of spectrum-eff ect relationship, which will lay a material basis for clarifying the antioxidant eff ects and controlling the quality of Rodgersia aesculifolia Batalin.
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