| 覃丽郦,白桂昌,张慧,何颂华.银翘解毒颗粒中金银花掺伪检查[J].中国药事,2021,35(12):1375-1382 |
| 银翘解毒颗粒中金银花掺伪检查 |
| Adulteration Inspection of Lonicerae Japonicae Flos in Yinqiaojiedu Granules |
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| DOI：10.16153/j.1002-7777.2021.12.008 |
| 中文关键词: 银翘解毒颗粒 金银花 山银花 灰毡毛忍冬皂苷乙 川续断皂苷乙 高效液相色谱-串联质谱法 |
| 英文关键词: Yinqiaojiedu Granules Lonicerae Japonicae Flos Lonicerae Flos macranthoidin B dipsacoside B LC-MS |
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| 中文摘要: |
| 目的： 建立银翘解毒颗粒中金银花掺伪检查方法，考察生产企业是否存在使用山银花代替金银花投料现象。方法：采用高效液相色谱-串联质谱法，色谱柱为Waters CORTECS(150 mm×4.6 mm， 2.7 μm)，以乙腈和水为流动相进行梯度洗脱，流速为0.5 mL·min-1。结果：灰毡毛忍冬皂苷乙在 0.0440~5.504 μg·mL-1呈良好的线性关系，平均回收率为101.49%，RSD=5.97%(n=9)；川续断皂苷乙在0.0458~5.720 μg·mL-1呈良好的线性关系，平均回收率为104.71%，RSD=4.99%(n=9)。结论：本方法灵敏、准确，可作为银翘解毒颗粒中金银花掺伪检查方法。 |
| 英文摘要: |
| Objective: To establish the identification method for the adulteration of Lonicerae Japonicae Flos in Yinqiaojiedu Granules to investigate whether there is a phenomenon of using Lonicerae Flos to replace Lonicerae Japonicae Flos in the manufacture enterprises. Methods: LC-MS method was used with Waters CORTECS (150 mm×4.6 mm, 2.7 μm) as analytical column, acetonitrile and water were used as mobile phase for gradient elution, the flow rate was 0.5 mL·min-1. Results: Macranthoidin B showed good linear relationship in the range of 0.0440-5.504 μg·mL-1, and the average recovery was 101.49% with RSD=5.97% (n=9). Dipsacoside B showed good linear relationship in the range of 0.0458-5.720 μg·mL-1, and the average recovery was 104.71% with RSD=4.99% (n=9). Conclusion: The method is sensitive and accurate, which can be applied to adulteration detection of Lonicerae Japonicae Flos in Yinqiaojiedu Granules. |
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