| 梁晟,杨青,李瑞莲.通迪胶囊中马兜铃酸Ⅰ测定方法的研究[J].中国药事,2021,35(7):751-756 |
| 通迪胶囊中马兜铃酸Ⅰ测定方法的研究 |
| Study on Determination Method of Aristolochic AcidⅠin Tongdi Capsules |
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| DOI：10.16153/j.1002-7777.2021.07.004 |
| 中文关键词: 通迪胶囊 马兜铃酸Ⅰ 超高效液相色谱 固相萃取 柱净化 限量检查 梯度洗脱 液质联用 |
| 英文关键词: Tongdi Capsules Aristolochic AcidⅠ UPLC solid-phase extraction column purification limited determination gradient elution LC-MS/MS |
| 基金项目:湖南省自然科学基金-科药联合基金项目(编号 2020JJ9032) |
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| 中文摘要: |
| 目的：建立通迪胶囊中马兜铃酸Ⅰ限量的 UPLC测定方法。方法：样品用70%甲醇提取，提取液通过Phenomenex Strata-X-AW(60 mg·3 mL-1)小柱除杂后用UPLC法进行测定。结果：马兜铃酸Ⅰ在0.149~4.957 μg·mL-1范围内线性关系良好(r2 =0.9994)，平均回收率为92.80%，RSD=2.21% (n=6)，7批样品中马兜铃酸Ⅰ的含量为4.56~8.94μg·g-1。结论：本法灵敏、快速、准确、专属性强，可用于通迪胶囊中马兜铃酸Ⅰ的限量检查。 |
| 英文摘要: |
| To establish a UPLC method for the determination of Aristolochic Acid Ⅰin Tongdi Capsules. Methods: Samples were extracted with 70% methanol and detected by UPLC after removing impurities from Phenomenex Strata-X-AW (60 mg·3mL-1) column. Results: The linearity range of Aristolochic Acid Ⅰ was from 0.149 to 4.957 μg·mL-1 (r2 =0.9994). The average recovery of Aristolochic Acid Ⅰwas 92.80% with the RSD=2.21% (n=6). The concentrations of Aristolochic Acid Ⅰin 7 batches of samples were 4.56-8.94 μg·g-1. Conclusion: The method is sensitive, rapid, accurate and specific enough to be used for the limited determination of Aristolochic Acid Ⅰ in Tongdi Capsules. |
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