| 王似锦,蔡春燕,余萌,刘鹏,杨美琴,马仕洪.屎肠球菌对复方胰酶散微生物计数中试验菌生长的影响[J].中国药事,2020,34(10):1186-1190 |
| 屎肠球菌对复方胰酶散微生物计数中试验菌生长的影响 |
| Effect of Enterococcus Faecium on the Growth of Test Strains in Microbial Counting for Compound Pancreatin Powder |
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| DOI：10.16153/j.1002-7777.2020.10.010 |
| 中文关键词: 屎肠球菌 复方胰酶散 微生物计数 |
| 英文关键词: enterococcus faecium Compound Pancreatin Powder microbial counting method |
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| 中文摘要: |
| 目的：研究屎肠球菌对复方胰酶散微生物计数方法适用性试验中试验菌生长的影响。方法：按照中国药典规定的方法适用性试验方法，分别对含有和不含屎肠球菌的复方胰酶散样品，用倾注法和涂布法进行试验，观察5种试验菌的生长情况，并计算回收率。结果：使用倾注法和涂布法，白色念珠菌和黑曲霉的回收率能够达到要求，使用涂布法(0.2 mL·皿-1)金黄色葡萄球菌、枯草芽孢杆菌和铜绿假单胞菌的回收率能够达到要求。结论：初步研究表明，使用倾注法时试验细菌的回收率偏低的原因是屎肠球菌的生长导致琼脂培养基pH值下降，从而抑制了加入的试验细菌的生长。 |
| 英文摘要: |
| Objective: To study the effect of enterococcus faecium on the growth of test strains in the suitability test of microbial counting method for Compound Pancreatin Powder. Methods: According to the suitability of the testing method prscribed by Chinese Pharmacopoeia, the samples of Compound Pancreatin Powder with or without enterococcus faecium were tested by Pour-plate method and Surface-spread method respectively. The growth of five test strains was observed, and the recovery rate was calculated. Results: The recovery rates of candida albicans and aspergillus niger could meet the requirements by Pour-plate method and Surface-spread method. The recovery rates of staphylococcus aureus, bacillus subtilis and pseudomonas aeruginosa could meet the requirements by Surface-spread method (0.2 mL·dish-1). Conclusion: The research suggested that the low recovery rate of test strains by Pour-plate method is due to the decrease of pH of agar medium caused by the growth of enterococcus faecium, which inhibits the growth of test bacteria. |
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