| 张荣林,薛亚馨,陆小康,马双成,昝珂,林雀跃,莫迎.加速溶剂萃取-高效液相色谱双波长法同时测定民族药广防风中3种成分的含量[J].中国药事,2020,34(3):335-341 |
| 加速溶剂萃取-高效液相色谱双波长法同时测定民族药广防风中3种成分的含量 |
| Determination of Three Compounds in Epimeredi indica (L.) Rothm. by An HPLC Dual Wavelength Method Combined with Accelerated Solvent Extraction |
| 投稿时间：2019-07-25 |
| DOI：10.16153/j.1002-7777.2020.03.014 |
| 中文关键词: 广防风 毛蕊花糖苷 异毛蕊花糖苷 木犀草素-7-O-β-D-葡萄糖醛酸苷 加速溶剂萃取 高效液相色谱法 |
| 英文关键词: Epimeredi indica (L.) Rothm. acteoside isoacteoside luteolin-7-O-β-D-glucuronide accelerated solvent extraction high performance liquid chromatography |
| 基金项目:科技部国家科技重大专项（民口）课题重大新药创制"中药组分资源库及产业公共技术服务平台建设"（编号2018ZX09735-006）；广西-东盟食品药品安全检验检测中心2019科研项目（编号KY201906） |
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| 中文摘要: |
| 目的： 建立加速溶剂萃取-高效液相色谱双波长法同时测定广防风中毛蕊花糖苷、异毛蕊花糖苷和木犀草素-7-O-β-D-葡萄糖醛酸苷3种活性成分的含量。方法： 通过优化加速溶剂萃取法的提取工艺，采用Ultimate XB-C18色谱柱（4.6 mm×250 mm，5μm），流动相为乙腈-0.1%甲酸溶液梯度洗脱，流速为1.0 mL·min-1，检测波长为330 nm和348 nm，柱温为35℃。结果： 优化得到的最佳萃取条件为以50%甲醇溶液25 mL （v/v）作为提取溶剂，于110℃，动态萃取4 min。毛蕊花糖苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、异毛蕊花糖苷进样量分别在0.02114~4.230 μg、0.02008~1.004μg、0.01979~1.979 μg范围内线性关系良好（r=1.0000），平均回收率分别为103.4%、99.7%、101.6%。结论： 本研究对广防风的质量标准提高具有较好的参考价值，为该药材的质量监管提供了先进的技术支持。 |
| 英文摘要: |
| Objective: To establish an accelerated solvent extraction-high performance liquid chromatography (HPLC) dual wavelength method for the simultaneous determination of the three active components——acteoside, isoacteoside and luteolin-7-O-β-D-glucuronide in Epimeredi indica (L.) Rothm. Methods: The primary parameters of accelerated solvent extraction, including extraction temperature, hold time and extraction solvent, were optimized. The separation was performed on an Ultimate XB-C18 column (4.6 mm×250 mm, 5μm), and the mobile phase consisted of acetonitrile and 0.1% formic acid with gradient elution. The detection UV wavelengths were 330 nm and 348 nm. The flow rate was 1.0 mL·min-1. The column temperature was 35℃. Results: The optimized conditions of accelerated solvent extraction were extraction solvent of 50% methanol 25 mL (v/v) with extraction temperature of 110℃, and hold time of 4 min. The calibration curves of acteoside, luteolin-7-O-β-Dglucuronide and isoacteoside showed good linearity over 0.02114-4.230 μg (r=1.0000), 0.02008-1.004 μg (r=1.0000), and 0.01979-1.979 μg (r=1.0000) respectively. The average recoveries were 99.7%-103.4%. Conclusion: This study provided a good reference for the improvement of quality standards of Epimeredi indica (L.) Rothm. and advanced technical support for the quality supervision of the medicinal substances. |
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