文章摘要
付海洋,苗雪文,姜爱莉,杨柳,王召旭.可降解丝素蛋白防粘连凝胶局部反应研究[J].中国药事,2019,33(7):783-789
可降解丝素蛋白防粘连凝胶局部反应研究
Study on Local Reaction of Degradable Fibroin Anti-adhesion Gel
投稿时间:2019-04-15  
DOI:10.16153/j.1002-7777.2019.07.010
中文关键词: 丝素蛋白防粘连凝胶  降解  皮下植入  局部反应
英文关键词: fibroin anti-adhesion gel  degradation  subcutaneous implantation  local reaction
基金项目:国家重点研发项目(编号2017YFC1105000)
作者单位E-mail
付海洋 烟台大学, 烟台 264000
中国食品药品检定研究院, 北京 102629 
 
苗雪文 烟台大学, 烟台 264000
中国食品药品检定研究院, 北京 102629 
 
姜爱莉 烟台大学, 烟台 264000  
杨柳 中国食品药品检定研究院, 北京 102629  
王召旭 烟台大学, 烟台 264000
中国食品药品检定研究院, 北京 102629 
wangzx@nifdc.cn 
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中文摘要:
      目的:通过可降解防粘连材料的皮下植入实验,分析材料降解过程中组织细胞的反应,评价可降解丝素蛋白防粘连凝胶局部组织相容性,探讨材料体内降解过程,为研究可降解防粘连材料体内降解机制提供支持。方法:将实验样品(丝素蛋白防粘连凝胶)植入新西兰家兔脊柱右侧皮下,对照样品(聚乳酸防粘连凝胶)植入新西兰家兔脊柱左侧皮下,于植入后1周、4周、12周和26周两侧取样进行组织病理学观察及降解情况研究,并对材料周围细胞进行分类计数和评价。结果:材料周围组织病理学显示,12周时实验样品组纤维囊厚度小于对照样品组;细胞分析结果显示,实验样品组1周、4周主要以淋巴细胞浸润为主,炎症反应高峰在4周,对照样品组1周、4周样品周围以巨噬细胞为主,12周时出现大量淋巴细胞和浆细胞,反应高峰在12周,26周时两组炎症细胞均降为0;半定量评价结果显示,与对照样品相比,丝素蛋白防粘连凝胶植入局部无明显刺激。结论:根据植入材料的局部反应情况,推测丝素蛋白防粘连凝胶的降解过程主要是以淋巴细胞为主的炎性浸润;聚乳酸防粘连凝胶4周前以巨噬细胞介导的呑噬反应为主,同时伴有炎症浸润,12周时转变为以淋巴细胞为主的炎性浸润。
英文摘要:
      Objective:To analyze the reaction of tissues and cells in the process of material degradation, evaluate the local histocompatibility of fibroin anti-adhesion gel, discuss the degradation process of the material in vivo, and to provide support for the study of the degradation mechanism in vivo of degradable anti-adhesion materials through the subcutaneous implantation experiment of degradable anti-adhesion materials. Methods:Test samples (fibroin anti-adhesion gel) were implanted into the right subcutaneous tissues of spine of New Zealand rabbits and the control samples (polylactic acid anti-adhesion gel) were implanted into the left subcutaneous tissues of spine of New Zealand rabbits. Histopathological observation and degradation study were carried out in the 1st week, the 4th week, the 12th week and the 26th week after implantation. Moreover, cells surrounding the materials were classified, counted and evaluated. Results:The histopathological results of tissues surrounding the materials showed that the thickness of the fibrous capsule in the test sample group was less than that of the control sample group in the 12th week after implantation. The results of the cell analysis showed that the test sample group was mainly infiltrated by lymphocytes in the 1st week and the 4th week after implantation and the peak of the inflammatory reaction was in the 4th week after implantation. The control group was mainly infiltrated by macrophages in the 1st week and the 4th week after implantation. A large number of lymphocytes and plasma cells appeared in the 12th week after implantation and the reaction peak was in the 12th week after implantation. Inflammatory cells in both groups dropped to zero in the 26th week after implantation. The semi-quantitative evaluation results showed that no obvious irritation was observed in the tissues implanted with fibroin antiadhesion gel compared with the control samples. Conclusion:According to the local reaction of the implanted materials, it is speculated that the degradation process of fibroin anti-adhesion gel is mainly inflammatory infiltration of lymphocytes. The degradation process of polylactic acid anti-adhesion gel is mainly macrophagemediated phagocytosis reaction, accompanied by inflammatory infiltration before the 4th week after implantation and transformed into lymphocytes-based inflammatory infiltration in the 12th week after implantation.
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