| 刘婷婷,姬俊,牛萌萌,张捷.中药饮片蒲黄、柴胡和青箱子中微生物污染情况考察[J].中国药事,2018,32(9):1220-1225 |
| 中药饮片蒲黄、柴胡和青箱子中微生物污染情况考察 |
| Investigation of Microbial Contamination in Traditional Chinese Medicine Decoction Pieces: Typhae Pollen, Bupleuri Radix and Celosiae Semen |
| 投稿时间：2018-05-19 |
| DOI：10.16153/j.1002-7777.2018.09.011 |
| 中文关键词: 中药饮片 微生物污染 需氧菌 耐热菌 耐胆盐革兰阴性菌 质量标准 |
| 英文关键词: traditional Chinese medicine decoction piece microbial contamination aerobic bacteria thermoduric bacteria bile-tolerant gram-negative bacteria quality standard |
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| 中文摘要: |
| 目的：考察3种中药饮片蒲黄、柴胡和青箱子中微生物污染状况。方法：每种饮片选取10个批次，进行需氧菌数、霉菌和酵母菌总数、耐热菌数、耐胆盐革兰阴性菌数、大肠埃希菌、沙门菌等检测。结果：饮片中需氧菌、霉菌和酵母菌、耐热菌的负载量很高，77%的饮片检出耐胆盐革兰阴性菌，43%检出大肠埃希菌，未检出沙门菌。经鉴定，3种饮片耐热菌主要为芽孢杆菌，耐胆盐革兰阴性菌主要为γ-变形菌纲细菌。结论：中药饮片微生物污染状况严重，饮片的微生物限度标准亟待完善，饮片生产加工过程控制亟待加强。 |
| 英文摘要: |
| Objective: To investigate the microbial contamination in traditional Chinese medicine decoction pieces, such as Typhae Pollen, Bupleuri Radix and Celosiae Semen.Methods: Ten batches of each decoction piece were detected for the numbers of aerobic bacteria, molds and yeasts, thermoduric bacteria, bile-tolerant gram-negative bacteria, Escherchia coli and Salmonella spp and so on.Results: The bioburden of aerobic bacteria, thermoduric bacteria, molds and yeasts in decoction pieces was high. The detection rates for bile-tolerant gram-negative bacteria and Escherchia coli were 77% and 43% respectively. Salmonella spp was not detected. Identification results showed that thermoduric bacteria in three traditional Chinese medicine decoction pieces were mainly Bacillus spp and bile-tolerant gram-negative bacteria were mainly γ-Proteobacteria.Conclusion: The microbial contamination in traditional Chinese medicine decoction pieces is serious. Microbial limit standard needs to be improved urgently and control of the production and processing of decoction pieces needs to be strengthened. |
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