| 黄元礼,柯林楠,王春仁.液相色谱-三重四级杆质谱联用法检测人类辅助生殖技术用液中N(2)-L-丙酰胺-L-谷氨酰胺含量[J].中国药事,2018,32(8):1097-1101 |
| 液相色谱-三重四级杆质谱联用法检测人类辅助生殖技术用液中N(2)-L-丙酰胺-L-谷氨酰胺含量 |
| The Determination of the Content of N(2)-L-alanyl-L-glutamine in In Vitro Fertilization Medium By UHPLC-MS/MS |
| 投稿时间：2017-07-07 |
| DOI：10.16153/j.1002-7777.2018.08.014 |
| 中文关键词: 体外生殖培养液 N(2)-L-丙氨酰-L-谷氨酰胺 超高效液相色谱-三重四级杆质谱联用法 质量控制 |
| 英文关键词: in vitro fertilization medium N(2)-L-alanyl -L-glutamine UHPLC-MS/MS quality control |
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| 中文摘要: |
| 目的：建立采用液相质谱联用法检测体外生殖培养液中N（2）-L-丙酰胺-L-谷氨酰胺指标成分的方法，分析不同用途培养液中N（2）-L-丙酰胺-L-谷氨酰胺的含量。方法：采用超高效液相色谱-三重四级杆质谱联用法，使用SUPELCO Discovery HS F5-3色谱柱（150 mm×2.1 mm，3 μm），流动相为0.1%甲酸水溶液-0.1%甲酸乙腈溶液，梯度洗脱，流速0.35 mL·min-1，柱温40℃；质谱采用电喷雾离子源，负离子模式扫描，扫描方式为多反应监测。结果与结论：N（2）-L-丙酰胺-L-谷氨酰胺在1.276~12.76μg·mL-1浓度范围内线性关系良好；平均加样回收率在100.5%~103.0%，相对标准偏差小于1.98%。说明采用液相质谱联用法检测体外生殖培养液灵敏、快速、准确、专属性强，可为体外生殖培养液的质量标准研究提供依据。 |
| 英文摘要: |
| Objective:To establish a method of determining N(2)-L-alanyl -L-glutamine in in vitro fertilization medium by UHPLC-MS/MS and to analyze the content of N(2)-L-alanyl -L-glutamine in fertilization medium for different purposes. Methods:UHPLC-MS/MS was used. The UHPLC separation was performed on a SUPELCO Discovery HS F5-3 column (150 mm×2.1 mm, 3 μm) in a gradient elute mode with acetonitrile and water (both containing 0.1% formic acid) as the mobile phase at a flow rate of 0.35 mL·min-1. The column temperature was 40℃. MS detection was performed with multiple reaction monitoring (MRM) mode by using negative electro spray ionization. Results and Conclusion:The linearity was achieved in the range of 1.276-12.76μg·mL-1 for N(2)-L-alanyl -L-glutamine. The average recovery rates were 100.5%-103.0% with RSD less than 1.98%, which indicates that the method is sensitive, rapid, accurate, and specific. It it will lay a basis for the study of quality standards of in vitro fertilization medium. |
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