| 兰钧,旺杰次仁,阿萍.高效液相色谱法测定藏药榜嘎中榜嘎苷A和榜嘎苷C的含量[J].中国药事,2018,32(7):906-912 |
| 高效液相色谱法测定藏药榜嘎中榜嘎苷A和榜嘎苷C的含量 |
| Determination of the Content of Bangga Flavonoid Glycoside A and Bangga Flavonoid Glycoside C in Tibetan Herb of Bangga by HPLC |
| 投稿时间：2017-10-26 |
| DOI：10.16153/j.1002-7777.2018.07.011 |
| 中文关键词: 榜嘎 榜嘎苷A 榜嘎苷C 高效液相色谱法 含量测定 |
| 英文关键词: Bangga Bangga flavonoid glycoside A Bangga flavonoid glycoside C HPLC determination of the content |
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| 中文摘要: |
| 目的：建立藏药榜嘎中榜嘎苷A和榜嘎苷C的HPLC含量测定方法。方法：采用Agilent XDB-C18（4.6 mm×250 mm，5 μm）色谱柱，以甲醇-乙腈（9：1）和0.4%磷酸为流动相，梯度洗脱，流速为1.0 mL·min-1，检测波长为325 nm，柱温为30℃。结果：榜嘎苷A进样量在0.1060~6.3621 μg线性关系良好（R2=1.0000），平均回收率为98.7%；榜嘎苷C进样量在0.1059~10.5878 μg线性关系良好（R2=0.9995），平均回收率为99.5%。结论：该方法经方法学验证，可用于藏药榜嘎的质量控制。 |
| 英文摘要: |
| Objective:To establish an HPLC method for the determination of the content of Bangga flavonoid glycoside A and Bangga flavonoid glycoside C in Tibetan herb of Bangga. Methods:When the chromatogram column was Agilent XDB-C18 (4.6 mm×250 mm,5 μm), the mobile phase consisted of methanol-acetonitrile (9:1) and 0.4% phosphoric acid with the gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was 325 nm and the column temperature was 30℃. Results:The calibration curve of Bangga flavonoid glycoside A had a good linear relationship in the sample range of 0.1060-6.3621 μg (R2=1.0000) and the average recovery rate was 98.7%. The calibration curve of Bangga flavonoid glycoside C also had a good linear relationship in the sample range of 0.1059-10.5878 μg (R2=0.9995) and the average recovery rate was 99.5%. Conclusion:The method was validated and could be used for the quality control of Tibetan herb of Bangga. |
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