| 昝珂,过立农,马双成,郑健.藏药独一味中30种化学成分的UPLC-ESI-TOF MS分析鉴定[J].中国药事,2018,32(6):757-763 |
| 藏药独一味中30种化学成分的UPLC-ESI-TOF MS分析鉴定 |
| Analysis of Thirty Chemical Constituents in Tibetan Medicine Lamiophlomis rotata by UPLC-ESI-TOF MS |
| 投稿时间：2016-10-11 |
| DOI：10.16153/j.1002-7777.2018.06.011 |
| 中文关键词: 独一味 化学成分鉴定 山栀苷甲酯 绿原酸 连翘酯苷B 芦丁 马钱苷 木犀草苷 毛蕊花糖苷 木犀草素 槲皮素 |
| 英文关键词: Lamiophlomis rotata identification of chemical constituents shanzhiside methyl ester chlorogenic acid forsythoside B rutin loganin luteoloside acteoside luteolin quercetin |
| 基金项目:十二五国家科技重大专项（编号2014ZX09304307-002） |
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| 中文摘要: |
| 目的：建立快速高效的方法，对藏药独一味的化学成分进行有效的鉴定，为该药材质量监督提供技术支持。方法：采用超高效液相-电喷雾-飞行时间质谱（UPLC-ESI-TOF MS）联用技术，色谱分离用ACQUITY UPLC HSS T3（2.1 mm×100 mm，1.8 μm），流动相组成比例分别为0.05%甲酸水和乙腈，梯度洗脱，流速0.3 mL·min-1，进样量1 μL；质谱定性采用飞行时间质谱，正离子模式扫描。结果：在优化的液质联用条件下，通过电喷雾-飞行时间质谱鉴定出独一味30个成分，分别为（1） phlomiol；（2） lamalbid；（3） phlorigidoside C；（4）胡麻属苷；（5）山栀苷甲酯；（6）5-羟基马钱苷；（7） vanillyl-O-β-D-葡萄糖苷；（8）绿原酸；（9）6-O-乙酰山栀苷甲酯；（10） cistanosideE；（11）7，8-dehydropenstemoside；（12） chlorotuberoside；（13）7-epi-loganin；（14）7-dehydroxy-2-zaluzioside；（15）马钱苷；（16）连翘酯苷B；（17）8-O-乙酰山栀苷甲酯；（18）芦丁；（19）木犀草苷；（20）毛蕊花糖苷；（21）木犀草素-7-O-β-D-呋喃芹糖基（1→6）-O-β-D-吡喃葡萄糖苷；（22） salviifoside A；（23）8-epi-7-deoxyloganin；（24）异类叶升麻苷；（25）芹菜素-7-O-β-D-葡萄糖苷；（26）6β-n-butoxy-7，8-dehydropenstemonoside；（27）芹菜素；（28）6-O-syringyl-barlerin；（29）木犀草素；（30）槲皮素。结论：通过UPLC-ESI-TOF MS联用技术，阐明了独一味的化学成分，为独一味及其制剂的质量控制和监管提供了科学依据；同时，为其他藏药的化学成分快速分析提供参考。 |
| 英文摘要: |
| Objective:To find out a rapid and efficient method to analyze effectively the chemical constituents in Tibetan medicine Lamiophlomis rotata and to provide technical support for the quality supervision of the medicinal materials. Methods:UPLC-ESI-TOF MS method was used and an ACQUITY UPLC HSS T3 (2.1 mm×100 mm, 1.8 μm) column was used for chromatographic seperation. The mobile phase consisted of water containing 0.05% formic acid and acetonitrile. The flow rate was 0.3 mL·min-1 and injection volume was 1 μL in gradient elution. TOF MS was applied for qualitative analysis under positive ion mode. Results:30 chemical constituents in Lamiophlomis rotata were identified under optimized LC-MS conditions, including phlomiol, lamalbid, phlorigidoside C, sesamoside, shanzhiside methyl ester, 5-hydroxy-loganin, vanillyl-O-β-Dglucopyranoside, chlorogenic acid, 6-O-acetyl shanzhiside methyl ester, cistanoside E, 7,8-dehydropenstemoside, chlorotuberoside, 7-epi-loganin, 7-dehydroxy-2-zaluzioside, loganin, forsythoside B, 8-O-acetyl shanzhiside methyl ester, rutin, luteoloside, acteoside, luteolin-7-O-β-D-apiofuranosy (1→6)-O-β-D-glucopyranoside, salviifoside A, 8-epi-7-deoxyloganin, isoacteoside, apigenin-7-O-β-D-glucopyranoside, 6β-n-butoxy-7,8-dehydropenstemonoside, apigenin, 6-O-syringyl-barlerin, luteolin, and quercetin. Conclusion:The chemical constituents in Lamiophlomis rotate were elucidated by UPLC-ESI-TOF MS. These results set up a scientific basis for the quality control and supervision of Lamiophlomis rotata and its preparations and provide references for the rapid analysis of chemical constituents in other Tibetan medicines. |
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