| 徐乐,孔亚萍,孙慧珠,刘永利.舒肝丸质量标准中薄层和液相方法完善研究[J].中国药事,2018,32(4):516-521 |
| 舒肝丸质量标准中薄层和液相方法完善研究 |
| Improvement Study on TLC and HPLC in Quality Standards of Shugan Pills |
| 投稿时间：2017-11-30 |
| DOI：10.16153/j.1002-7777.2018.03.015 |
| 中文关键词: 舒肝丸 薄层色谱法 高效液相色谱法 质量标准完善 芍药苷 |
| 英文关键词: Shugan Pills TLC HPLC improvement of the quality standard paeoniflorin |
| 基金项目: |
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| 中文摘要: |
| 目的：对舒肝丸质量标准进行完善提高。方法：对现标准中延胡索、陈皮、厚朴的薄层色谱鉴别方法进行完善提高；采用高效液相色谱法同时测定芍药苷的含量，色谱柱为C18柱，以乙腈-0.05%磷酸为流动相，梯度洗脱，流速1.0 ml·min-1；检测波长为230 nm。结果：薄层色谱斑点清晰，分离度好，专属性强，重复性良好。芍药苷在0.03898~0.9746μg范围内线性关系良好；平均回收率为100.5%，RSD为1.5%。结论：本文建立的鉴别和含量测定方法为舒肝丸的质量标准的修订完善提供可靠依据。 |
| 英文摘要: |
| Objective: To improve the quality standards for Shugan Pills. Methods: The TLC identification methods of corydalis rhizoma, citri reticulatae pericarpium, magnoliae offcinalis cortex in the current quality standard were optimized. The content of paeoniflorin was determined by HPLC. The chromalographic separation was performed on a C18 column with acetonitrile -0.05% phosphoric acid solution with gradient elution as the mobile phase. The flow rate was 1.0ml·min-1 and the detection wavelength was 230nm. Results: The chromatographic spots were clear with good separation, and the chromatogram for identification by TLC was specific and highly repeatable. The calibration curve was linear within the range of 0.03898~0.9746μg for paeoniflorin. The average recovery was 100.5% and RSD was 1.5%. Conclusion: The established identifcation and content determination methods in this paper provide a reliable basis for the revision and improvement of the quality standards of Shugan Pills. |
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