| 邢俊生,张彬,李倩.食品虾仁中土霉素、四环素、金霉素含量测定方法的建立[J].中国药事,2017,(10):1199-1204 |
| 食品虾仁中土霉素、四环素、金霉素含量测定方法的建立 |
| Establishment of the Method to Determine Terramycin, Tetracycline and Chlorotetracycline in Shrimp Meat |
| 投稿时间：2016-04-29 |
| DOI：10.16153/j.1002-7777.2017.10.018 |
| 中文关键词: 食品安全监测 土霉素 四环素 金霉素 高效液相色谱 梯度洗脱 |
| 英文关键词: food safety monitoring Terramycin Tetracycline Chlorotetracycline HPLC gradient elution |
| 基金项目: |
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| 中文摘要: |
| 目的：建立高效液相色谱法测定虾仁中土霉素、四环素、金霉素的含量。方法：采用C18色谱柱（250 mm×4.6 mm，5 μm）；梯度洗脱，以甲醇为流动相A，乙腈为流动相B，10 mmol·L-1三氟乙酸为流动相C；流速1.5 mL·min-1；检测波长为350 nm。结果：土霉素、四环素、金霉素检出限分别为0.005、0.005和0.020 mg·kg-1，土霉素、四环素线性范围为0.05~1.00 μg·mL-1，相关系数为0.9996及0.9995，金霉素线性范围为0.20~4.00 μg·mL-1，相关系数为0.9998；加标回收率为73.5%~94.2%。结论：本方法简便、灵敏、专属，重复性好，可用于同时测定虾仁中土霉素、四环素、金霉素的含量。 |
| 英文摘要: |
| Objective: To establish an HPLC method to determine Terramycin, Tetracycline and Chlorotetracycline in shrimp meat. Methods: The column was C18 (250 mm×4.6 mm, 5μm). The gradient elution program was used with a flow rate of 1.5 mL·min-1 and UV detection at 350 nm. Mobile phase A was methyl alcohol; mobile phase B was acetonitrile; mobile phase C was 10 mmol·L-1 trifluoroacetic acid. Results: The detection limit of Terramycin, Tetracycline and Chlorotetracycline was 0.005 mg·kg-1, 0.005 mg·kg-1 and 0.020 mg·kg-1, respectively. The linear range of Terramycin and Tetracycline was 0.05-1.00 μg·mL-1. The correlation coefficients were 0.9996 and 0.9995, respectively. The linear range of Chlorotetracycline was 0.20-4.00 μg·mL-1, The correlation coefficient was 0.9998. The recovery rate was 73.5%~94.2%. Conclusion: This method was simple, sensitive, exclusive and reproducible, which could be used for simultaneous determination of Terramycin, Tetracycline and Chlorotetracycline in dried shrimp meat. |
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