| 过立农,郑凰雅,刘杰,马双成,昝珂,郑健.菥蓂HPLC指纹图谱的建立和2种碳苷成分的含量测定[J].中国药事,2017,(9):1032-1038 |
| 菥蓂HPLC指纹图谱的建立和2种碳苷成分的含量测定 |
| Establishment of HPLC Fingerprint Chromatogram of Thlaspi arvense L. and Content Determination of Two Flavone C-glycosides |
| 投稿时间：2016-12-08 |
| DOI：10.16153/j.1002-7777.2017.09.013 |
| 中文关键词: 菥蓂 高效液相色谱 指纹图谱 特征峰 异荭草苷 异牡荆苷 含量测定 |
| 英文关键词: Thlaspi arvense L. HPLC fingerprint chromatogram characteristic peaks isoorientin isovitexin content determination |
| 基金项目:十二五国家科技重大专项（编号2014ZX09304307-002） |
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| 中文摘要: |
| 目的：采用高效液相色谱法建立菥蓂的指纹图谱，并同时测定2种主要黄酮碳苷的含量。方法：以异荭草苷和异牡荆苷为对照品，采用HSS T3色谱柱（250 mm×4.6 mm，5 μm）；以乙腈-0.5%醋酸水溶液（15：85）为流动相，流速1 mL·min-1，检测波长350 nm，柱温30℃；采用CHEMPATTERN化学计量学软件对结果进行处理分析。结果：建立菥蓂的指纹图谱，标定5个特征峰，30 min内菥蓂的主要色谱峰能够达到完全分离，对2个特征峰成分进行含量测定。13批菥蓂中异荭草苷和异牡荆苷的含量分别为0.015%~0.094%和0.034%~0.323%。结论：建立了菥蓂的指纹图谱，结合2种主要碳苷含量测定，为菥蓂药材的质量评价提供科学依据。 |
| 英文摘要: |
| Objective:To establish fingerprint chromatogram using the method of HPLC and to determine two main flavone C-glycosides in Thlaspi arvense L. Methods:Isoorientin and isovitexin were used as reference substance. HPLC analysis was performed on a Waters HSS T3 column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.5% acetic acid solution (15:85) with isocratic elution and the flow rate was 1 mL·min-1. The detection wavelength was 350 nm and the column temperature was 30℃. The results were analyzed using the Chemometrics software Chempattern. Results:HPLC fingerprint chromatogram of Thlaspi arvense L. was established and five characteristic peaks were marked. The main chromatographic peaks of Thlaspi arvense L.were completely separated in 30 minutes. Two characteristic peaks of isoorientin and isovitexin were simultaneously determined by HPLC. The contents of the two flavones C-glycosides in 13 samples of Thlaspi arvense L. were 0.015%-0.094%, and 0.034%-0.323%, respectively. Conclusion:The established HPLC fingerprint chromatogram together with content determination of two flavones C-glycosides can be used for the quality control of the crude drug. |
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