| 姜雪敏,杨立志.小儿吐泻宁质量标准中含量测定研究[J].中国药事,2017,(8):940-943 |
| 小儿吐泻宁质量标准中含量测定研究 |
| Study on the Assay Method for Xiaoertuxiening Quality Standard |
| 投稿时间：2016-08-18 |
| DOI：10.16153/j.1002-7777.2017.08.018 |
| 中文关键词: 中药制剂 质量标准 小儿吐泻宁 厚朴酚 和厚朴酚 高效液相色谱法 |
| 英文关键词: Chinese medicine preparations quality standard Xiaoertuxienin particles Magnolol Honokiol HPLC |
| 基金项目: |
|
| 摘要点击次数: 753 |
| 全文下载次数: 506 |
| 中文摘要: |
| 目的：小儿吐泻宁的质量标准中无含量测定项目，本文建立小儿吐泻宁中厚朴酚与和厚朴酚的含量测定方法，为该药品的质量标准修订提供参考。方法：采用高效液相色谱法，ZORBAX SBC18色谱柱，以甲醇-水（70：30）为流动相，检测波长为294 nm，流速为1.0 mL·min-1。结果：厚朴酚进样量在0.0403~0.8056μg内与峰面积具有良好的线性关系（r=1.0000）、和厚朴酚进样量在0.0604~1.2074μg内与峰面积具有良好的线性关系（r=1.0000），厚朴酚的平均回收率为97.8%，RSD=1.5%；和厚朴酚的平均回收率为98.0%，RSD=1.0%。厚朴酚含量为1.43 mg·袋-1，和厚朴酚含量为4.19 mg·袋-1。结论：本文建立的方法符合方法学验证要求，可为小儿吐泻宁的质量标准含量测定项目修订提供参考。 |
| 英文摘要: |
| Objective: There is no determination in quality standard of Xiaoer tuxiening. In this paper, we set up a method for determination of magnolol and honokiol to provide references for the revision of the quality standard of this medicine. Methods: To establish a method by HPLC, the separation was performed on a ZORBAX SB-C18 column by using Methanol-Water (70:30) as mobile phase. The UV detection was set at 294nm; flow rate was 1.0mL·min-1. Results: The magnolol was in good linearity between the peak area and injection volume in the ranges of 0.0403~0.8056 μg (r=1.0000); The honokiol was in good linearity between the peak area and injection volume in the ranges of 0.0604~1.2074 μg (r=1.0000); The average recovery of magnolol was 97.8%, RSD=1.5%; The average recovery of honokiol was 98.0%, RSD=1.0%. The content of magnolol was 1.43mg·package-1, and honokiol content was 4.19mg·package-1. Conclusion: The method meets its validation requirements, and can provide references for the revision of the quality standard of Xiaoertuxienin. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |