| 宁霄,金绍明,刘雅丹,曹进,丁宏.超高效液相串联质谱法同时测定保健食品中10种水溶性维生素[J].中国药事,2017,(4):392-402 |
| 超高效液相串联质谱法同时测定保健食品中10种水溶性维生素 |
| Simultaneous Detection of 10 Kinds of Water-soluble Vitamins in Health Food Using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry |
| 投稿时间：2016-10-29 |
| DOI：10.16153/j.1002-7777.2017.04.008 |
| 中文关键词: 保健食品 超高效液相色谱-串联质谱(UPLC-MS/MS) 水溶性维生素 同时测定 |
| 英文关键词: health food ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) water-soluble vitamins simultaneous detection |
| 基金项目: |
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| 摘要点击次数: 1052 |
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| 中文摘要: |
| 目的：建立超高效液相色谱-串联质谱检测方法同时测定保健食品中10种水溶性维生素。方法：样品经过前处理后，采用Waters ACQUITY UPLC HSS T3（2.1×；100 mm，1.8μm）色谱柱分离；以0.1%甲酸的甲醇溶液和0.1%甲酸的水溶液为流动相进行梯度洗脱，通过电喷雾离子源多反应监测（MRM）模式进行检测。结果：10种水溶性维生素在6 min内完成分离，线性关系良好（r≥0.997），方法检出限在5~250μg·kg-1之间。3个添加水平的回收率和相对标准偏差RSD（n=6）分别为85.9%~109.5%和1.09%~0.79%。结论：该方法简便、快速、准确、灵敏，适用于保健食品中水溶性维生素的测定。 |
| 英文摘要: |
| Objective: To develop an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous detection of 10 kinds of water-soluble vitamin in health food. Methods: The samples were pretreated, separated by using a Waters ACQUITY UPLC HSS T3(2.1mm×100 mm, 1.8μm) column, followed by gradient elution with 0.1% formic acid in methanol and 0.1% formic acid solution as the mobile phase, and were detected with electrospray ionization in multiple reaction monitoring (MRM) mode. Results: The 10 kinds of water-soluble vitamin samples were separated within 6 minutes. The method showed good linear relationship (r≥0.997) and detection limit was between 5 and 250μg·kg-1. The recoveries and RSD (n=6) of this method were 85.9%-109.5% and 1.09%-6.79%, respectively. Conclusion: The method is simple, rapid, accurate, sensitive and suitable for the detection of water-soluble vitamins in health food. |
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