文章摘要
续艳丽,祁景琨,续创业,徐雪梅.藏药六味木香丸的定性定量检测方法研究[J].中国药事,2017,(3):295-300
藏药六味木香丸的定性定量检测方法研究
On the Qualitative and Quantitative Detection Methods of Tibetan Medicine Liuwei Muxiang Wan
投稿时间：2016-08-25  
DOI：10.16153/j.1002-7777.2017.03.011
中文关键词: 藏药  六味木香丸  木香  石榴子  余甘子  荜茇  木香烃内酯  去氢木香烃内酯  定性检测  定量分析  质量标准
英文关键词: Tibetan medicine  Liuwei Muxiang Wan  Radix Aucklandiae  pomegranate seed  Phyllanthi Fructus  Piperis Longi Fructus  costunolide  dehydrocostuslactone  qualitative detection  quantitative analysis  quality standard
基金项目:
作者单位E-mail
续艳丽 甘肃省兰州市食品药品检验所, 兰州 730000  
祁景琨 中国食品药品检定研究院, 北京 100050  
续创业 平凉市农业科学院, 平凉 744000  
徐雪梅 甘肃省兰州市食品药品检验所, 兰州 730000 516246899@qq.com 
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中文摘要:
      目的： 建立藏药六味木香丸的定性定量检测方法，为质量标准修订提供参考。方法：采用TLC法对藏药六味木香丸中木香、石榴子、余甘子和荜茇进行定性鉴别；采用HPLC法测定藏药六味木香丸中木香烃内酯和去氢木香烃内酯的含量，使用C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-水（65∶35）为流动相，流速1.0 mL·min-1，检测波长225 nm，柱温25℃。结果：薄层色谱斑点清晰，分离度良好，阴性样品无干扰。木香烃内酯质量浓度在12~60 μg·mL-1范围内线性关系良好（r=0.9997），平均回收率（n=6）为98.8%，RSD=1.6%；去氢木香烃内酯质量浓度在17~85 μg·mL-1范围内线性关系良好（r=0.9996），平均回收率（n=6）为99.8%，RSD=1.4%。结论：所建立的方法准确、可靠，专属性强，可用于六味木香丸的质量控制。
英文摘要:
      Objective: To establish the qualitative and quantitative detection methods for Tibetan Medicine Liuwei Muxiang Wan. Methods: TLC was used to identify Radix Aucklandiae, Pomegranate seed, Phyllanthi Fructus, and Piperis Longi Fructus. HPLC method was used to determine costunolide and dehydrocostuslactone in Tibetan Medicine Liuwei Muxiang Wan. The procedure was performed on an Agilent 5 TC-C18 column (4.6 mm×250 mm, 5 μm) at 25℃, with acetonitrile-water (65:35) as mobile phase and the detection wavelength was set at 225 nm. Results: The clear TLC spots showed good resolution without interference from negative samples. The calibration curve of costunolide was linear in the mass concentration range of 12-60 μg·mL-1 (r=0.9997), and the average recovery rate was 98.8% with the corresponding RSD of 1.6%; the calibration curve of dehydrocostuslactone was linear in the mass concentration range of 17-85 μg·mL-1 (r=0.9996), and the average recovery rate was 99.8% with the corresponding RSD of 1.4%. Conclusion: The established method of identification is accurate, simple and specific, and can be used in the quality control of Liuwei Muxiang Wan.
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