| 蒋秋香,吴柳春.壮药金钮扣定性定量方法研究[J].中国药事,2017,(3):290-294 |
| 壮药金钮扣定性定量方法研究 |
| On the Qualitative and Quantitative Method for Quality Control of Zhuang Medicine Spilanthes paniculata Wall. ex DC. |
| 投稿时间：2016-05-23 |
| DOI：10.16153/j.1002-7777.2017.03.010 |
| 中文关键词: 民族药 壮药 金钮扣 绿原酸 TLC HPLC |
| 英文关键词: national medicine Zhuang medicine spilanthes paniculata chlorogenic acid TLC HPLC |
| 基金项目: |
|
| 摘要点击次数: 945 |
| 全文下载次数: 385 |
| 中文摘要: |
| 目的： 建立金钮扣药材的定性定量质量控制方法，考察广西不同产地、不同采收时节金钮扣的药材质量。方法：以乙酸丁酯-乙酸乙酯-甲酸-水（11∶3∶1.5∶1.5）为展开剂，采用TLC法对金钮扣进行定性鉴别。用C18色谱柱（4.6 mm×250 mm，5 μm），柱温30℃，以乙腈-0.4%磷酸溶液（10∶90）为流动相，流速1.0 mL·min-1，检测波长为327 nm，采用HPLC法对金钮扣中的绿原酸进行含量测定。结果：薄层板上斑点清晰，分离度好；绿原酸在2.032~25.40μg·mL-1（r=0.9995）线性关系良好；加样回收率（n=6）为100.9%，RSD为1.2%，所测得的13批样品含量在0.042~0.741 mg·g-1之间。结论：该方法简便、准确，重复性好，可用于壮药金钮扣药材的质量控制。 |
| 英文摘要: |
| Objective: To establish a qualitative and quantitative method for quality control of Spilanthes paniculata Wall. ex DC., and investigate the quality of this medicine in Guangxi at different habitats and harvest time. Methods: The TLC method was used for qualitative identification with butyl acetate-ethyl acetate-formic acid-water (11:3:1.5:1.5) being developing agent. The content determination of chlorogenic acid was conducted by HPLC by using a C18 column (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.4% phosphoric acid (10:90) at a flow rate of 1.0 mL·min-1, the detection wavelength was 327 nm, and the column temperature was 30℃. Results: The spot of chlorogenic acid could be well identified by TLC. In the system of HPLC, chlorogenic acid showed good linear relationship in the range of 2.032-25.40 μg·mL-1(r=0.9995). The recovery rate was 100.9% (n=6), RSD=1.2%. The contents of the 13 batches of samples were between 0.042 and 0.741 mg·g-1. Conclusion: The method is simple, accurate, reliable and can be used for the quality control of Spilanthes paniculata Wall. ex DC. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |