| 王珺,韩晓萍,卜晨琛.HPLC法筛查百花定喘制剂中前胡投料[J].中国药事,2017,(3):281-289 |
| HPLC法筛查百花定喘制剂中前胡投料 |
| Screening of Peucedanum praeruptorum in Baihua Dingchuan Preparations by HPLC |
| 投稿时间：2016-09-01 |
| DOI：10.16153/j.1002-7777.2017.03.009 |
| 中文关键词: 百花定喘制剂 前胡 紫花前胡 白花前胡甲素 白花前胡乙素 紫花前胡苷 HPLC |
| 英文关键词: Baihua Dingchuan Preparations Peucedanum praeruptorum Peucedanum decursivum Praeruptorin A Praeruptorin B Nodakenin HPLC |
| 基金项目: |
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| 摘要点击次数: 795 |
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| 中文摘要: |
| 目的： 通过测定白花前胡甲素、白花前胡乙素和紫花前胡苷的含量，建立筛查百花定喘制剂中投料为前胡还是紫花前胡的HPLC方法。方法：采用Symmentry-C18（4.6 mm×250 mm，5 μm）色谱柱，流动相：甲醇（A）-0.1%磷酸溶液（B），梯度洗脱，检测波长为321 nm，流速为1.0 mL·min-1。结果：5批药材的标示名称与试验判断结果不一致；47批百花定喘制剂的筛查结果表明，未见紫花前胡投料，前胡存在投料不足的情况。结论：该方法专属性良好，可作为定性筛查白花定喘制剂中投料前胡的方法。 |
| 英文摘要: |
| Objective: To develop an HPLC method for the determination of Praeruptorin A, Praeruptorin B and Nodakenin, so as to screen Peucedanum praeruptorum or Peucedanum decursivum in Baihua Dingchuan Preparations. Methods: The separation was performed with Waters Symmentry-C18(4.6 mm×250 mm, 5 μm) column. A gradient elution of methanol and 0.1% phosphoric acid solution were adopted as the mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was 321 nm. Results: The 5 batches of medicinal materials were inconsistent with experimental result. In addition, Peucedanum decursivum was not found in 47 batches of Baihua Dingchuan Preparations, but Peucedanum praeruptorum was in shortage. Conclusion: The established method was specific, reliable and simple, which is adoptable for quality control of Peucedanum praeruptorum in Baihua Dingchuan Preparations. |
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