| 高丽梅,郑健.HPLC法测定蒙药巴日嘎-13散中大叶茜草素的含量[J].中国药事,2017,(2):178-181 |
| HPLC法测定蒙药巴日嘎-13散中大叶茜草素的含量 |
| Determination of Mollugin in Mongolian Medicine Bariga-13 Powder by HPLC |
| 投稿时间：2015-03-29 |
| DOI：10.16153/j.1002-7777.2017.02.012 |
| 中文关键词: 蒙药 高效液相色谱法 巴日嘎-13散 大叶茜草素 |
| 英文关键词: Mongolian medicine HPLC Bariga-13 mollugin |
| 基金项目: |
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| 中文摘要: |
| 目的：建立高效液相色谱法测定巴日嘎-13散中茜草有效成分大叶茜草素的含量。方法：DikmaDimonsil C18色谱柱（250 mm×4.6 mm，5 μm），流动相为甲醇-水-四氢呋喃（527：90：3），检测波长为391nm，流速为1 mL·min-1。结果：大叶茜草素在5.5~55 μg·mL-1范围内呈良好的线性关系（r=0.9999），平均加样回收率为102.24%，RSD为1.48%。结论：本方法简便可靠、分离度较好、结果稳定，可用于巴日嘎-13散的质量检测。 |
| 英文摘要: |
| Objective: To develop a method for determination of mollugin in a prepared Mongolia traditional medicine Bariga-13.Methods: HPLC method was built by using Dikma Dimonsil Diamonsil C18 (250 mm×4.6 mm, 5μm) as column and methanol-water-tetrahydrofuran (527:90:3) as mobile phase. The detection wavelength was set at 391 nm.Results: The calibration curve of Mollugin was linear (r=0.9999) in the range of 5.5-55 μg·mL-1. The average recovery was 102.24%; RSD was 1.48%.Conclusion: The method is simple, reproducible and reliable to produce good separation results. It can be applied to the quality test of Bariga-13. |
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