| 陈岳蓉,李普衍,刘海青.藏药七味诃子散中有效成分丁香酚的含量测定[J].中国药事,2017,(2):165-169 |
| 藏药七味诃子散中有效成分丁香酚的含量测定 |
| Determination of Eugenol in Qiwei Hezi Powders |
| 投稿时间：2016-08-31 |
| DOI：10.16153/j.1002-7777.2017.02.009 |
| 中文关键词: 七味诃子散 丁香酚 丁香化学成分 藏族验方 民族药质量标准 |
| 英文关键词: Qiwei Hezi powders eugenol chemical composition of Caryophylli Flos Tibetan prescriptions ethnodrug quality standard |
| 基金项目: |
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| 中文摘要: |
| 目的：建立七味诃子散中丁香酚含量的HPLC测定方法。方法：采用Phenomenex Luna 5 μ C18色谱柱（4.6 mm×250 mm，5 μm），流动相为甲醇-水（55：45），流速为1 mL·min-1，检测波长为柱温为25℃。结果：丁香酚的进样量在0.564~2.820 μg范围内线性关系良好（r=0.9999），平均回收率为99.03%（RSD=1.01%）；样品中丁香酚的含量测定结果为14.7~15.6 mg·g-1。结论：本方法可用于测定七味诃子散中丁香酚的含量，可为该药质量标准修定提供参考。 |
| 英文摘要: |
| Objective: To develop an HPLC method for determination of eugenol in Qiwei Hezi powders.Methods: The optimal HPLC conditions were as follows:Phenomenex Luna 5 μ C18 column (4.6 mm×250 mm, 5 μm) was adopted, methanol-water (55:45) was used as mobile phase, flow rate was 1 mL·min-1, detection wavelength was 280 nm, and column temperature was 25℃.Results: There was a good linearity between peak areas and injection quantity of eugenol within the range of 0.564-2.820 μg (r=0.9999). The average recovery was 99.03% (RSD=1.01%). The contents of eugenol in Qiwei Hezi powders were 14.7-15.6 mg·g-1.Conclusion: The method can detect eugenol in Qiwei Hezi powders, and can provide references for the quality standard revision of this drug. |
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