文章摘要
才让草,潘遐,李魁,才让三知,高晓昱,张骞.藏药石榴莲花散中鞣花酸和胡椒碱含量测定方法研究[J].中国药事,2020,34(2):215-221
藏药石榴莲花散中鞣花酸和胡椒碱含量测定方法研究
On Content Determination of Ellagic Acid and Piperine in Tibetan Medicine Pomegranate Lotus Powder
投稿时间：2019-05-22  
DOI：10.16153/j.1002-7777.2020.02.010
中文关键词: 藏药  石榴莲花散  鞣花酸  胡椒碱
英文关键词: Tibetan medicine  pomegranate lotus powder  ellagic acid  piperine
基金项目:甘肃省食品药品监督管理局科研项目（编号2018GSFDA029）
作者单位E-mail
才让草 甘南藏族自治州药品检验检测中心, 甘南 747000  
潘遐 定西市药品检验检测中心, 定西 743000 107271403@qq.com 
李魁 甘南藏族自治州药品检验检测中心, 甘南 747000  
才让三知 甘南藏族自治州藏医医院, 甘南 747000  
高晓昱 定西市药品检验检测中心, 定西 743000  
张骞 甘南藏族自治州食品检验检测中心, 甘南 747000  
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中文摘要:
      目的： 建立藏药石榴莲花散中鞣花酸和胡椒碱含量测定方法，以完善其质量标准。方法： 采用高效液相色谱法测定制剂中石榴子和诃子的鞣花酸含量，采用ODS-2 HYPERSIL C18色谱柱（4.6 mm×250mm，5 μm），以乙腈-0.2%磷酸（15：85）为流动相，流速1.0 mL·min-1，检测波长254 nm，柱温30℃；采用高效液相色谱法测定制剂中荜茇的胡椒碱含量，采用ODS-2 HYPERSIL C18色谱柱（4.6mm×250 mm，5μm），以乙腈-水（40：60）为流动相，流速1.0 mL·min-1，检测波长343 nm，柱温30℃。结果： 鞣花酸进样量在5.065~25.325 μg范围内呈良好线性关系（r=0.9999），平均回收率为97.5%，RSD=1.5%（n=6）；胡椒碱进样量在10.414~52.071 μg范围内呈良好线性关系（r=1.0000），平均回收率为99.6%，RSD=0.6%（n=6）。结论： 该方法可准确测定石榴莲花散中鞣花酸和胡椒碱的含量，有助于提高石榴莲花散的质量标准。
英文摘要:
      Objective: To establish a method for the content determination of ellagic acid and piperine in pomegranate lotus powder, a Tibetan medicine, in order to improve its quality standard. Methods: The content of ellagic acid in pomegranates and chebula was determined by High Performance Liquid Chromatography (HPLC). The column was ODS-2 HYPERSIL C18 (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.2% phosphoric acid (15:85), the detection wavelength was 254 nm, the flow rate was 1.0 mL·min-1, and the column temperature was 30℃. HPLC was used to determine piperine of long pepper in the preparation. The column was ODS-2 HYPERSIL C18 (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrilewater (40:60), the flow rate was 1.0 mL·min-1, the detection wavelength was 343 nm, and the column temp erature was 30℃. Results: There was a good linear relationship (r=0.9999) between 5.065 and 25.325 μg for ellagic acid, and the average recovery was 97.5% with RSD of 1.5% (n=6). There was a good linear relationship (r=1.0000) between 10.414 and 52.071 μg for piperine, with an average recovery of 99.6% and RSD of 0.6% (n=6). Conclusion: This method could accurately determine the content of ellagic acid and piperine in pomegranate lotus powder, which was helpful to improve the quality standard of pomegranate lotus powder.
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