| 查祎凡,聂黎行,于健东,戴忠,马双成.化学发光法测定中药抗氧化活性研究进展[J].中国药事,2019,33(1):32-40 |
| 化学发光法测定中药抗氧化活性研究进展 |
| Research Progress of Determination of Antioxidant Activity of Traditional Chinese Medicine by Chemiluminescence Method |
| 投稿时间：2018-01-04 |
| DOI：10.16153/j.1002-7777.2019.01.006 |
| 中文关键词: 化学发光 中药 抗氧化 自由基 |
| 英文关键词: chemiluminescence traditional Chinese medicine antioxidant free radical |
| 基金项目:国家自然科学基金（编号81303194）；中国食品药品检定研究院学科带头人培养基金（编号2017X1）；国家中医药管理局"中药标准化建设"项目（编号ZYBZH-C-JS-32） |
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| 中文摘要: |
| 抗氧化活性是中药的重要药效之一，化学发光法（CL）是有力的评价手段，具有灵敏度高、线性范围宽、重现性好等优点。本文对近10年来化学发光法在中药抗氧化活性评价中的应用进行了综述，涉及多种中药材和中成药，包括直接测定和流动注射法（FIA）测定自由基清除率，与毛细管色谱（CE）和高效液相色谱（HPLC）联用筛选抗氧化活性成分，并辅以质谱鉴定结构。最后，展望化学发光法在中药质量评价中的应用前景。 |
| 英文摘要: |
| Antioxidant activity is one of the important pharmaceutical effects of traditional Chinese medicine (TCM). Chemiluminescence (CL) is a better evaluation method with high sensitivity, wide linear range and good reproducibility. In this paper, the application of CL in the evaluation of antioxidant activity of TCM in recent 10 years was reviewed, involving a variety of Chinese medicinal materials and Chinese patent mecdicines. Direct assay and flow injection analysis (FIA) were used to determine free radical scavenging rate. Capillary electrophoresis (CE) was combined with high performance liquid chromatography (HPLC) to screen antioxidant active ingredients. The structure was identified by mass spectrometry. Finally, application of CL in the quality evaluation of TCM was prospected. |
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